一株人乳头瘤病毒阳性的新的食管癌细胞系的建立及鉴定  

Establishment and Identification of A New HPV Positive Esophageal Cancer Cell Line

作　　者：李劲涛[1,2] 周福有[3] 董温平[2] 王立东[4] 曾毅[2,1] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052 [3]河南安阳市肿瘤医院,安阳455000 [4]郑州大学医学院,郑州450052

出　　处：《病毒学报》2013年第2期119-125,共7页Chinese Journal of Virology

基　　金：传染病预防控制国家重点实验室自主研究重点课题(2011SKLID103);国家科技支撑计划项目(No.2006BAI19B03)

摘　　要：为了探讨人乳头瘤病毒(Human papillomavirus,HPV)与上消化道肿瘤食管癌关系,从食管癌高发区安阳市取到一例76岁的中国女性食管鳞癌患者肿瘤组织。通过直接SCID小鼠致瘤实验,可长成移植瘤。取出组织做组织培养并多次纯化传代筛选后,成均一单层细胞。分别进行免疫荧光,细胞生长曲线,软琼脂鉴定,染色体分析,细胞致瘤实验及瘤体切片HE染色等确定是上皮细胞来源的癌细胞。利用细胞STR分型结果显示,基因座均未出现三等位基因现象,无人类细胞交叉污染。对细胞DNA分析发现存在HPV18型的DNA。利用蛋白检测实验发现有HPV病毒癌蛋白表达。结果表明,所建细胞株为有HPV核酸存在并能表达病毒癌蛋白的新的食管鳞癌细胞株。为我们进行食管癌的发生发展的病因学研究提供了新的细胞学材料。In order to explore the relationship between human papilloma virus （HPV） and upper gastroin- testinal cancer（esophageal cancer）, An esophageal squamous cell carcinoma（ESCC） tissue was obtained from a 76 year old Chinese female patient from Anyang city, a high-incidence area for esophageal cancer, in China. Transplanted tumor was formed through direct SCID mouse tumorigenicity experiment and cultured monolayer cells were obtained after several passages and screenings Immunofluorescence test, cell growth curve, soft agar assay, chromosome analysis and tissues HE staining were also performed to confirm the epithelial cell origin. Cell DNA STR typing results showed that no three alleles was observed,indicating no contamination of human cells. DNA analysis revealed the presence of HPV type 18 DNA in this cell line. DOLINK test found the E6 protein expression of HPV virus. We concluded that the established cell line is a new esophageal squamous cell-origincarcinoma cell line with HPV DNA positive and expression of viral oncoprotein. It provides new cytologic material for performing etiology studies on the occurrence and development of esophageal cancer.

关 键 词：HPV 食管癌 细胞系 

分 类 号：R730.261[医药卫生—肿瘤]