羊瘙痒因子263K感染仓鼠临床终末期脑中αB-晶体蛋白表达的研究  

Analysis of The Expressions of αB-crystallin in The Brain Tissues of Agent 263K-infected Hamsters at Terminal Stage

作　　者：王轲[1,2] 任科[2] 燕玉娥[2] 王荟[2] 张宝云[2] 刘勇[1] 董小平[2] 张瑾[2] 

机构地区：[1]西安交通大学医学院,西安710061 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《病毒学报》2013年第2期192-196,共5页Chinese Journal of Virology

基　　金：国家自然科学基金青年项目(81100980);传染病预防控制国家重点实验室面上项目(2011SKLID204);传染病预防控制国家重点实验室留学归国人员启动经费(2011SKLID302)资助

摘　　要：αB-晶体蛋白(αB-crystallin)是小热休克蛋白(Small heat shock protein,sHSP)家族的代表成员,已发现与多种神经退行性疾病发生发展密切相关,但至今为止对αB-晶体蛋白在朊病毒病发生发展中可能扮演的角色研究甚少,作用尚不清楚。本研究利用羊瘙痒因子仓鼠敏感株263K感染仓鼠建立的朊病毒实验动物模型,通过免疫印迹(Western blots)和免疫组织化学染色观察到伴随PrPSc在感染终末期动物脑组织中大量沉积,αB-晶体蛋白表达显著上调,比正常对照增高3倍。免疫荧光双染确定其分布的主要细胞类型为星形胶质细胞,神经元中未检测到表达。感染动物中高表达的αB-晶体蛋白与异常沉积的PrPSc无明显共定位。此研究为进一步探讨和揭示αB-晶体蛋白在朊病毒病中可能的作用和分子机制奠定了基础。αB-crystallin is a tant role in multiple neuro member of the sHSP （Small h degeneration diseases. To give of αB-crystallin in prion disease, the aB-crystallin levels eat shock protein） family, which plays an impor-insight into the possible alternation and the role in the brain tissues of agent 263K-infected hamsters were evaluated. Western blots and IHC revealed that at the terminal stages of the diseases, the levels of αB-crystallin were increased up to 3-fold in the brain tissues of scrapie infected 263K hamsters compared with normal controls. Immunofluorescent assays revealed that the up-regulated αB-crystallin was mainly observed in astrocytes, but not in neurons. The co-localization between ;B-crystallin and abnormal deposition of PrPsc in the brain tissues of the serapie infected hamsters was not observed. The study may provide a foundation for further revealing the potential role of αB-crystallin in prion disease.

关 键 词：朊病毒病 αB-晶体蛋白 星型胶质细胞 PRPSC 

分 类 号：R373.9[医药卫生—病原生物学]