2010～2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析  

Molecular Characterization and Phylogenetic Analysis of Porcine Epidemic Diarrhea Virus Field Strains in Central China during 2010-2012 Outbreaks

作　　者：郑逢梅[1] 霍金耀[1] 赵军[1] 常洪涛[1] 王晓梦[1] 陈陆[1] 王川庆[1] 

机构地区：[1]河南农业大学,郑州450002

出　　处：《病毒学报》2013年第2期197-205,共9页Chinese Journal of Virology

摘　　要：2010年底开始,猪流行性腹泻(Porcine epidemic diarrhea,PED)在中国境内出现严重暴发。为探讨该病突然再次暴发的原因,对2010年10月至2012年6月于华中地区11个地市收集的12株猪流行性腹泻病毒(Porcineepidemic diarrhea virus,PEDV)S、M和ORF3基因进行RT-PCR扩增、克隆及测序,并对其序列变化、遗传进化关系、抗原位点、糖基化位点和多肽极性进行分析。结果显示,12株PEDV流行毒株S、M和ORF3基因部分核苷酸及氨基酸的改变使其关键序列明显不同于以往毒株,其中有11株流行毒株的S基因比现用疫苗毒株CV777多9个核苷酸,而且在其S1区N′端存在大量的氨基酸突变。遗传进化分析显示,12株PEDV华中地区毒株与2007～2009年韩国毒株、2007～2008年泰国毒株、2009～2010年越南毒株、2011年日本毒株及2010～2012年中国其它地区流行毒株亲缘关系均较密切,而与欧洲株(CV 777、Br1/87)、中国现用疫苗株(CV777)及2003～2007年中国分离株(LJB/03、JS-2004-2、DX、QH、LZC)亲缘关系均较远。与现用疫苗株(CV777)相比,流行毒株S蛋白的抗原位点、糖基化位点和跨膜螺旋均有明显改变,提示PEDV在近年呈现快速变异和进化的趋势,因而可能需要选择更有效的疫苗株来控制PEDV的暴发。Since late 2010, porcine epidemic diarrhea virus （PEDV） has been re-emerging in central China. To explore the possible reason of the PEDV outbreaks, twelve PEDV field strains were isolated from dif- ferent swine breeding farms in central China during 2010-2012, and molecular diversity, phylogenetic relationships of these strains with other PEDV reference strains were investigated. Sequence analysis of S, M and ORE3 genes revealed that the central China PEDV isolates had several specific nucleotides and amino acids which were different from PEDV reference strains. In addition, the entire S genes of eleven central China PEDV isolates were found to be nine nucleotides longer in length than CV777 and large number of amino acid variations was accumulated in the N-terminal region of S gene. Phylogenetic analysis showed that the central China PEDV isolates had close relationship with Korea strains （2007-2009）, Thailand strains （2007-2008）, Vietnam strains （2009-2010）, Japan strains （2010）, and other prevailing strains from other parts of China （2010-2012）. However, they differed genetically from European strains （CV777, Brl/87）, China strains （2003-2007） and the vaccine strains （CV777） used in China. These results imply that a rapid variation and evolution of central China;IPEDV strains has occurred in recent years, and a more efficient vaccine strain should be selected to prevent and control outbreaks of PEDV in China.

关 键 词：猪流行性腹泻病毒 分子特征 系统进化分析 S基因 ORF3基因 M基因 病毒变异 

分 类 号：S852.65[农业科学—基础兽医学]