中国人群eNOS基因内含子13微卫星多态性的初步研究  

Preliminary investigation of the microsatellite at the endothelial nitric oxide synthase gene intron 13 in Chinese

作　　者：林琳[1] 李月琴[1] 谢卫兵[1] 姚冬生[1] 海戎[1] 云泓若[1] 周天鸿[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2000年第4期20-23,共4页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：目的 :为研究eNOS基因内含子 13上的微卫星序列与妊高症的相关性。方法 :采用PCR-PAGE与银染相结合的方法 ,研究其多态性。结果 :得到含有该微卫星序列的DNA片段。结论 :证实在中国人群中eNOS基因内含子 13上的确存在一个CA重复的多态性微卫星序列 ,从而为研究该位点与妊高症的相关性提供了技术支持和理论依据。Aim:To investigae the relation between the microsatellite of eNOS gene intron 13 and pregnancy-induced hypertension (PIH), the polymorphism of this microsatellite is first studied. Methods: the polymorphism is studied by PCR-PAGE and silver-stained method. Results: the fragment including microsatellite sequence is obtained. Conclusion: the results showed that there is a microsatellite-polymorphism at eNOS gene intron 13 in Chinese, this would provide support and theoretical base for further analysis of the relation between this polymorphism and PIH. [

关 键 词：内含子13 CA重复 ENOS基因 微卫星序列 多态性 

分 类 号：Q78[生物学—分子生物学] Q987.1