GDF-9和BMP-15在不同品系小鼠中的多态性和生殖能力关联分析  

Polymorphism identification in mice GDF9 and BMP15 genes and their association analysis with litter size

作　　者：殷昆仑[1,2] 张长勇[2] 王天奇[2] 暴国[2] 付淑霞[2] 何嘉玲[2] 孙德明[2] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]国家人口计生委科学技术研究所,北京100081

出　　处：《中国比较医学杂志》2013年第3期8-13,I0001,共7页Chinese Journal of Comparative Medicine

基　　金：国家科技支撑项目课题(2012BA131B06)

摘　　要：目的研究GDF-9和BMP-15在繁殖能力不同的品系小鼠ICR和BALB/c中的多态性,为筛选相关的模型动物种群提供参考资料。方法采用直接测序法筛查多态性位点,并对多态性位点构建双荧光素酶报告基因载体,检测5’UTR对下游基因的影响。结果 ICR小鼠的产仔数是BALB/c的2.8倍左右;在ICR和BALB/c之间发现了GDF-9存在三个多态性位点,一个位于5’端的非编码区,另外两个位于蛋白编码区;双荧光素酶检测结果显示GDF-9的5’UTR区可以促进下游基因的表达。BMP15在两种品系中不存在差异性位点。结论 GDF-9在ICR和BALB/c这两个品系小鼠中存在多态性位点,这些位点的变化对下游基因的表达起一定的促进作用。BMP15在ICR和BALB/c小鼠中不存在多态性位点。Objective To study the polymorphism of GDF29 and BMP-15 in mice of different strains, different reproductive capacity, for screening related model animal population provide reference material. Methods Screening polymorphism loci by using direct sequencing method, and testing 5＇UTR impact on downstream genes by constructing luciferase dual reporter vector. Result ICR mice litter size is about 2.8 times the BALB/c; In ICR and BALB/c, respectively, found three polymorphisms, one at the 5＇end of the non-coding region, the other two located in the protein coding region; Dual-luciferase test results show that the 5＇ UTR area of GDF-9 can promote the expression of the downstream gene. BMP15 in two strains are not present in the difference site. Conclusions GDF-9 polymorphic loci exist in ICR and BALB/c strains, changes in these sites play a certain role in promoting the expression of the downstream gene.

关 键 词：GDF-9 BMP-15 小鼠 繁殖力 基因多态性 作用机制 

分 类 号：R-332[医药卫生]