丹酚酸A对实验性肺纤维化大鼠的保护作用研究  

Protection effect of Salvianolic acid A on pulmonary fibrosis of rat

作　　者：孔勤[1] 刘瑞敏[1] 寿旗扬[1] 肖颖[1] 朱科燕[1] 陈民利[1] 

机构地区：[1]浙江中医药大学动物实验研究中心比较医学研究中心,杭州310053

出　　处：《中国比较医学杂志》2013年第3期34-38,I0003,共6页Chinese Journal of Comparative Medicine

基　　金：浙江省卫生高层次创新人才培养工程项目

摘　　要：目的观察丹酚酸A对博来霉素诱导的肺纤维化大鼠的保护作用及可能机制。方法气管单次给予博来霉素注射液复制大鼠肺纤维化模型,第2天开始给予丹酚酸A,连续28天,记录体重与一般体征变化;处死大鼠,测定血清自由基、肺功能及观察组织透射电镜等情况。结果造模后给予丹酚酸A可改善肺纤维化大鼠一般体征状况;提高血清SOD、GSH-Px活性和降低MDA含量;改善肺功能;减轻肺泡炎症,缩小肺泡间隔宽度;电镜观察发现给予肺纤维化大鼠丹酚酸A后,胶原纤维生成减少,Ⅱ型肺泡上皮细胞增生减弱。结论丹酚酸A对博来霉素诱导的实验性肺纤维化有一定的保护作用,其机制可能与丹酚酸A抗氧化作用有关。Objective Investigate the effects of salvianolic acid A on rats of bleomycin-induced pulmonary fibrosis and potential mechanisms. Methods Single-dose bleomycin was given via intratracheal on Day 0 and rats were treated from Dayl daily with tail-vein injection of salvianolic acid A for 28 d. Record weights and observe general physical sign. Measure free radical,pulmonary function and take electron microscopes. Results Salvianolic acid A could recover physical sign, enhance SOD and MDA activities and reduce MDA contents. Salvianolic acid A could minimize of alveolar spaces and alleviate the inflammation. Moreover, it could improve pulmonary function and decrease collagen expression, inhibits type II alveolar epithelial cells hyperplasia in pulmonary fibrosis rats. Conclusion Salvianolic acid A could attenuate the bleomycin induced pulmonary fibrosis of rats and potential mechanism may be correlated with antioxidation of SAA.

关 键 词：丹酚酸A 肺纤维化 抗氧化 保护 

分 类 号：R-332[医药卫生]