Expression of Plasminogen Activator Inhibitor-2 is Negatively Associated with Invasive Potential in Hepatocellular Carcinoma Cells  

作　　者：Ye Jin Li Zhou Ke-min Jin Bao-cai Xing 

机构地区：[1]Clinical Research Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College [2]Department of General Surgery,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences & Peking Union Medical College [3]Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Department of Hepatobiliary Surgery I,Peking University Cancer Hospital

出　　处：《Chinese Medical Sciences Journal》2013年第1期16-19,共4页中国医学科学杂志（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China (863 Program,2006AA02A308);the National Key Technologies Research and Development Program of China (2008ZX10002-016);the Beijing Municipal Key Project(H030230280410)

摘　　要：Objective To investigate the association between plasminogen activator inhibitor(PAI)-2 expression and invasive potential in hepatocellular carcinoma(HCC) cells.Methods The HCC cell lines with high,low,and non-metastatic potentials,namely MHCC97-H,MHCC97-L,and SMMC-7721 respectively,were cultured in vitro.Matrigel invasion assay and Western blot of PAI-2 protein expression were conducted.Results The number of invaded cells in MHCC97-L was significantly higher than that in SMMC-7721(P=0.005),whereas that in MHCC97-H was higher than in MHCC97-L(P=0.017) and SMMC-7721(P=0.001).Contrarily,PAI-2 protein expression was gradually reducing from SMMC-7721,MHCC97-L,to MHCC97-H(MHCC97-H vs.MHCC97-L,P<0.001;MHCC97-H vs.SMMC-7721,P=0.001;MHCC97-L vs.SMMC-7721,P=0.001).The Pearson's correlation analysis revealed a significant negative association between invaded cell number and PAI-2 expression(r= 0.892,P=0.001).Conclusion PAI-2 expression may be negatively associated with the invasive potential of HCC.

关 键 词：hepatocellular carcinoma plasminogen activator inhibitor-2 invasive potential 

分 类 号：R735.7[医药卫生—肿瘤]