他达拉非增加小鼠脑实质淋巴瘤微血管通透性的研究  

Tadanarll increases microvascular permeability in mouse brain lymphoma models

作　　者：陈文立[1] 王嵘[2] 张强[2] 乔小云[2] 刘勇[2] 瞿叶清[2] 朱学军[3] 

机构地区：[1]中山大学附属第一医院黄埔院区神经外科,广州510700 [2]南京大学附属鼓楼医院 [3]江苏省中医院

出　　处：《中华神经外科杂志》2013年第3期299-302,共4页Chinese Journal of Neurosurgery

基　　金：南京市医药卫生科研课题（YKK10065）

摘　　要：目的研究他达拉非增加脑淋巴瘤内微血管通透性。方法采用依文思蓝检测通透性、高效液相法检测氨甲喋呤浓度、以及电镜观察超微结构对比分析他达拉非对小鼠脑实质淋巴瘤模型脑微血管通透性的影响。结果他达拉非组脑淋巴瘤血管通透性相较其他各组有显著增加（P〈0．05）。氨甲喋呤在他达拉非组脑淋巴瘤组织中浓度增加近1．8倍，显著高于其他各组（P〈0．05）。他达拉非组小鼠脑淋巴瘤脑微血管内皮细胞内电镜下见大量微囊液泡。结论磷酸二酯酶抑制剂他达拉非可以显著增加小鼠脑淋巴瘤微血管的通透性。Objective To study about Tadalafil increasing microvascular permeability in mouse brain lymphoma models. Methods Comparatively analyze the influence of tadalafil on cerebral microvascular permeability in the mouse brain lymphoma models, by the approaches of using Evan＇s Blue to test the microvascular permeability, testing concentrations of methotrexate （MTX） by HPLC, and studying ultrastructures by transmission electron microscope. Results The microvascular permeability in tumor - bearing mice treated with Tadalafil were significantly higher than that in the other groups （ P 〈 0. 05）. The concentration of MTX increased nearly 1. 8 times in tumor tissue with Tadalafit pre - treatment. The increment were significantly higher than that in the other groups （ P 〈 0. 05）. Numerous pinocytotic vesicles in the cytoplasm of the endothelial ceils were observed obviously in Tadalafil pre - treated mouse tumor. Conclusion Phosphodiesterase type 5 inhibitor, Tadalafil, could increased the capillary permeability of the tumor tissue in mouse brain lymphoma models.

关 键 词：脑微血管 他达拉非 依文思蓝 氨甲喋呤 淋巴瘤 

分 类 号：R739.4[医药卫生—肿瘤]