锰离子对兔眼视网膜毒性的形态学研究  

Toxicity of Manganese on Rabbit Retina

作　　者：王玮玲[1,2] 马志中[1] 李颖[1] 任秋实[3] 孙晓东[4] 胡运韬[1] 

机构地区：[1]北京大学第三医院北京大学眼科中心,北京100083 [2]宁夏医科大学总医院眼科,银川750004 [3]北京大学生物医学工程系,北京100083 [4]上海交通大学附属第一人民医院眼科,上海200080

出　　处：《宁夏医科大学学报》2013年第1期4-8,F0002,共6页Journal of Ningxia Medical University

基　　金：国家"973"项目(2011CB707506)

摘　　要：目的观察玻璃体腔注射不同浓度Mn2+对兔眼视网膜的毒性,从而筛选出合理的玻璃体腔注射浓度,为临床应用和实验研究提供依据。方法 36只青紫兰兔,随机分为9组,其中8个实验组分别于单眼玻璃体内注入不同浓度MnCl2溶液(0.5、1、2、5、10、15、20、40mmol.L-1)25μL,对照组单眼注射同容积生理盐水,均于注药7d后取材,光学显微镜、电子显微镜观察评价Mn2+对眼视网膜组织结构的毒性作用。结果 1)玻璃体腔注入0.5～15mmol.L-1MnCl27d后,视网膜外节盘膜完整,排列规则,各级神经元之间的排列正常,细胞核染色质分布均匀,超微结构未发现明显异常;2)当浓度﹥20 mmol.L-1病理损伤渐明显。结论注射剂量25μL,浓度﹤15mmol.L-1的MnCl2溶液是青紫兰兔玻璃体腔注射的安全剂量。Objective To investigate the toxic effect of 0.5 - 40 mmol/L Mn^2 ＋ on rabbit retina after intravitreal injection, and to verify the safe dose of intravitreal injecting MnC12. Methods Thirty - six pigment tabbits were divided at random into 9 groups. A single dose of MNC12（25 μL） with the concentrations of 0.5, 1, 2, 5, 10, 15, 20, 40 mmol·L^-1 were intravitreally injected separately in 8 groups, and the normal rabbitswere used as control group. Retinal toxicity was examined by light microscope and transmission electron microscopy （TEM） on the seventh day after injection. Results Histological examinations showed that the themorphology of retina was normal in 0.5, 1, 2, 5, 10 mmol·L^-1 MnC12 group on the seventh day after injection. No obvious cellular degeneration was seen in various layers of retina in 20 mmol·L^-1 MnC12 group onthe seventh day after injection,however, in 40 mmol·L^-1 MnC12 group, the disorder of retinal structure, disappearance of outer plexiform layer, inordinance of cells arrangement in internal granular layer were seen un-der the light microscope. TEM examination revealed mild RPE cells abnormality in 20 mmol·L^-1 MnC12 group, but pyknosis of RPE cells, loss of disc membrane, nonintact of outer limiting membrane were exhibitedin 40 mmol·L^-1 MnC12 group under the TEM on the seventh day after injection. Conclusion This study indicate that the safe dose of MnC12 as a tracer of optical pathway via intravitreal injection is 〈 15 mmol·L^-1.

关 键 词：锰离子 毒性 视网膜 

分 类 号：R774.1[医药卫生—眼科]