射线照射对腮腺机能与分泌细胞胞膜影响的观察  

Changes of the salivary function and the fragility of the membrane of the salivary secreted cell after unilateral parotids radiation by high energy X-ray

作　　者：刘英杰[1] 胡国清[2] 张春盈[1] 许克璞[1] 赵静[1] 苏静[1] 王峻峰[2] 肖志平[2] 黄河[2] 

机构地区：[1]解放军第152中心医院肿瘤综合治疗中心,河南平顶山467000 [2]华中科技大学同济医学院附属同济医院肿瘤中心,湖北武汉430030

出　　处：《中华肿瘤防治杂志》2013年第7期493-496,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:了解单侧腮腺高能射线照射,对双侧唾液淀粉酶、总蛋白、唾液分泌量以及分泌细胞膜脆性影响。方法:大梅杂交猪6头,4头双侧腮腺导管造瘘后单侧腮腺照射作为照射组,对侧未照射腮腺为自身对照组;2头双侧腮腺单纯造瘘不照射为阴性对照组。放疗开始后定时收集6头造瘘动物唾液31d,观察初次照射30min内和24h后脱落细胞酸溶血变化。检测双侧唾液分泌量、淀粉酶、总蛋白各16次,采用SPSS 11.0软件处理数据q检验比较各指标差异情况。结果:实验组、自身对照组和阴性对照组唾液量分别为(16.63±13.12)、(39.03±13.14)和(23.52±6.01)mL,淀粉酶活性均值分别为(13 696.58±4 587.10)、(15 692.12±3 523.13)和(14 821.32±3 163.47)U/dL,差异均有统计学意义,P<0.01。总蛋白分别为(9.75±0.54)、(9.23±0.54)和(9.05±0.45)g/dL,差异无统计学意义,P>0.05。初次照射后30min内、24h后双侧唾液内细胞膜脆性增加。结论:单侧腮腺照射,可影响同侧、对侧唾液淀粉酶和唾液分泌量以及唾液内细胞胞膜脆性。OBJECTIVE：To investigate the changes of salivary amylase, total protein, secretory volume, fragility of the secreted cells in hibateral parotids after radiation of unilateral salivary glands. METHODS： Salivary ducts was fistulized on both sides of the 4 among the 6 Damei Hybridization swine,radiation was administered on unilateral salivary glands,as radiated group. The contralateral parotids were taken as self-control group. The other 2 swines were merely fistulized, as negative-control group. Once the radiation begins, sialon of the 6 swine was collected timely for 31 d, Ham＇s test was performed on the secreted cell within 30 min and 24 h after the initial radiation. Hibateral salivary amylase,total protein, secretory volume all for 16 times, q-test of SPSS 1l. 0 was used to show the difference of each index. RESULTS： Salivary quantities were （16.63±13.12） ,（39.03±13.14） and （23.52±6.01） mL in radiated group,self-control group,negative group,respectively. Activities of amylase were （13 696. 58 ± 4 587. 10）, （15 692. 12 ± 3 523. 13） and （1 4821. 32± 3 163.47） U/dL （P〈0. 01）. While total proteins were （9.75±0.54）,（9.23±0. 54） and （9.05±0. 45） g/dL （P〈 0.05）. The fragility of hibateral secreted cell membrane increases within the 30 min and Z4h after the initial radiation. CONCLUSION：Unilateral salivery glands radiation may effect bilareral hibateral salivary amlysase, sialon volume and fra- gility of sialon secreted cell.

关 键 词：唾液腺 辐射 淀粉酶类 胞膜脆性 

分 类 号：R739.63[医药卫生—肿瘤]