肿瘤标志物DJ-1、NSE、CYFRA21-1和CEA联合检测在肺癌诊断中的应用价值  

The value of combined detection of tumor markers DJ-1,NSE,CYFRA21-1 and CEA in diagnosis of lung cancer

作　　者：陈婧[1] 汪宏良[1] 程晖[1] 吴琴[1] 尹义军[1] 

机构地区：[1]黄石市中心医院检验科,湖北黄石435000

出　　处：《国际检验医学杂志》2013年第6期651-652,655,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨肿瘤标志物DJ-1蛋白(DJ-1)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段21-1(CYFRA21-1)及癌胚抗原(CEA)在肺癌患者血清中的水平以及在肺癌诊断中的价值。方法采用酶联免疫吸附法(ELISA)检测血清DJ-1蛋白,采用电化学发光法(ECLIA)检测血清NSE、CYFRA21-1和CEA水平,比较分析肺癌组、肺部良性疾病组和健康对照组的各60例病例。结果肺癌组的4种血清肿瘤标志物水平均高于肺部良性疾病组和健康对照组,差异有统计学意义(P<0.01);肺癌病理类型中鳞癌中有较高的DJ-1和CYFRA21-1水平,腺癌中有较高的CEA水平,而小细胞癌中有较高的NSE水平;以上4种肿瘤标志物联合检测肺癌的诊断价值最高,灵敏度为85%,特异度为78.3%,约登指数达到0.63。结论 DJ-1、NSE、CYFRA21-1及CEA联合检测对肺癌的诊断有一定的价值,可以作为高危人群初步筛查的指标。Objective To study the serum level of DJ-1,neuronspeeific enolase（NSE） ,eytokeratin 19 fragment 21-1（CYFRA21- 1） ,carcino-embryonic antigen（CEA） in cases and their value in diagnosis of lung cancer. Methods Serum DJ-1（with enzyme-linked immunosorbent assay）,NSE,CYFRA21-1,CEA（with electrochemiluminescence）level were detected in 60 patients with lung cancer, 60 patients with pulmonary infection and 60 healthy controls . Results 4 serum tumor markers in patients with lung cancer were significant higher than those in patients with pulmonary infection and healthy controls （P（0.01）;the sensitivity, specificity and Youden index of DJ-1,NSE,CYFRA21-1 ,CEA combined detection were 85% ,78.3% and 0.63 in lung cancer. Conclusion DJ-1, NSE, CYFRA21-1 and CEA levels have significance in detection of lung cancer, which provide evidence for diagnosis of lung cancer. Combined detection with 4 tumor markers can improve sensitivity and specificity in diagnosis of lung cancer.

关 键 词：肺肿瘤 肿瘤标记 生物学 诊断 DJ-1蛋白 

分 类 号：R734.2[医药卫生—肿瘤]