大鼠全脑缺血/再灌注对海马区p63和Bcl-2表达的影响  

Effects of global cerebral ischemia/reperfusion on p63 and Bcl-2 expression of hippocampus in rats

作　　者：廖常莉[1] 周述之[1] 张英[1] 赵晓蓉[1] 欧册华[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《山东医药》2013年第12期8-11,I0001,I0002,共6页Shandong Medical Journal

基　　金：四川省卫生厅科研项目(100289);泸州医学院自然科学基金项目(2011-24)

摘　　要：目的探讨大鼠全脑缺血/再灌注对海马CA1区p63和Bcl-2表达的影响。方法 80只SD大鼠随机分为假手术组(Sham组)和全脑缺血/再灌注组(I/R组),每组40只。两组分别在手术后6、12、24、48、72 h处死8只大鼠,进行神经行为学评分,检测p63、Bcl-2蛋白表达、神经元密度及凋亡情况。结果 I/R组p63阳性细胞率较Sham组同时相增高(P均<0.05),组内48 h最高(P<0.01);I/R组细胞凋亡指数较Sham组增加(P均<0.05),其中术后48 h最高(P<0.05);I/R组Bcl-2平均灰度值较Sham组低(P均<0.01),组内术后6 h开始降低、12 h增加、24 h最高(P<0.01)。结论全脑缺血/再灌注后p63与Bcl-2蛋白表达呈先增加后减少的趋势,与神经元凋亡趋势基本一致,提示p63与Bcl-2可能参与了脑神经元的调控。Objective To observe the effects of global cerebral ischemia/reperfusion on 1363 and Bcl-2 expression of hippocampus in rats. Methods 80 male SD rats were randomly divided into two groups： Sham group and global cerebral ischemia/reperfusion （I/R） group, 40 in each group. Rats were executed for cerebral tissues at 6, 12, 24, 48 and 72 h after operation, 8 in each time point. Neuroethology was evaluated. The expressions of p63 and Bel-2 protein, neuronal density and apoptosis in hippocampus CA1 area were detected. Results The positive ratio of the cells expressing 1363 pro- tein in I/R group was significantly higher than that in sham group at the same time point （ P 〈 0.05 ） , the ratio of 48 h was higher than that of 24 h and 72 h （ P 〈 0.01 ）. Apoptosis indexes at 24 h, 48 h and 72 h after operation in I/R group were significantly increased compared with the Sham group （ P 〈 0.05 ）, maximum at 48 h. The average gray value of Bcl-2 in I/ R group was significantly lower than that in Sham group at the same time point （P 〈0.01 ）, and decreased at 6 h, in- creased at 12 h, maximum at 24 h （P 〈 0.01 ）. Conclusion The expressions of p63 and Bcl-2 in global cerebral I/R showed a decreasing trend after the first increase, consistent with neuronal apoptosis trend, suggesting that p〈5.3 and Bcl-2 might be involved in the regulation of brain neurons.

关 键 词：脑缺血 P63 BCL-2 神经元凋亡 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]