暗褐网柄牛肝菌菌丝深层发酵培养试验  

Studies on Submerged Fermentation of Phlebopus portentosus Mycelium

作　　者：王文兵[1] 张春霞[1] 何明霞[1] 刘静[1] 纪开萍[1] 

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带农业科技》2013年第2期28-30,共3页Tropical Agricultural Science & Technology

基　　金：国家自然科学基金资助项目(31060271);农业科技成果转化资金项目(2011GB2F300004);云南省农垦集团项目(298)

摘　　要：深层发酵培养是扩大菌种的主要方式之一,采用摇瓶法对暗褐网柄牛肝菌(Phlebopus portentosus)适宜的碳源、氮源、发酵条件进行研究,筛选出适合暗褐网柄牛肝菌生长的最佳碳源为甘露醇,其菌丝生物量平均为40.3 g/L,最佳氮源为酵母膏,其菌丝生物量平均为95.8 g/L;发酵条件为:震荡速度130 r/min,培养温度28℃,接种量在250 mL三角瓶装液量100 mL的情况下为1.62cm2,培养周期10 d。Submerged fermentation is one of the main ways of expanding strains. Suitable carbon source, nitrogen source, fermen- tation conditions of Phlebopus portentosus were studied using the shake flask method. The results show that the best carbon source is mannitol, mycelia biomass average for 40.3g / L. The best nitrogen source is yeast extract, mycelia biomass average for 95.8g / L. The fermentation conditions are as follows： the shaker rotation speed is 130r/min, the temperature is 28 ℃, the opti- mal culture media capacity is 100ml in 250ml-flask, mycelium block is 1.62cm2, culture period is 10 days.

关 键 词：暗褐网柄牛肝菌 深层发酵 菌丝体 

分 类 号：S567.3[农业科学—中草药栽培]