创伤性脑损伤脑组织NF-kB表达变化规律及PDTC对其影响  

The effect of PDTC on the expression change of nuclear factor-kappaB after experimental traumatic rats brain injury

作　　者：刘盛明[1] 苑玉清[1] 侯敏[1] 吴虹[1] 

机构地区：[1]成都市第三人民医院神经外科,四川成都610031

出　　处：《四川医学》2013年第2期167-169,共3页Sichuan Medical Journal

摘　　要：目的探讨急性创伤性脑损伤后核转录因子-kB(NF-kB)表达变化规律,以及NF-kB特异性抑制剂PDTC能否减轻继发性脑损伤,以期为治疗颅脑损伤提供新策略。方法将Wistar大鼠随机分为3组:假手术对照组、脑损伤组及脑损伤治疗组。以免疫组织化学法观察创伤性脑损伤后NF-kB表达的变化规律及NF-kB抑制剂PDTC对脑损伤后NF-kB表达的影响。结果在脑损伤组动物的脑组织内,围绕损伤灶神经变性区,从伤后6h到120h,NF-kB表达较假手术对照组明显增高。而PDTC脑损伤治疗组,NF-kB表达较损伤组明显下降(P<0.05)。结论创伤后NF-kB表达显著增高,NF-kB激活可能是继发性脑损害的重要分子机制。PDTC可能通过抑制NF-kB表达,从而对创伤性脑损伤后继发性脑损害具有保护作用。Objective To investigate the expression of NF-kB after traumatic brain injury,and if the secondary brain injury would decrease when the expression of NF-kB was inhibited by PDTC,in order to provide the theoretical basis directly for a new approach to the clinical management of traumatic brain injury.Methods The free-falling-body impact device was used to cause the models of local moderate contusion and laceration in the rats.Sixty Wistar rats were randomly divided into three groups： control group,injury group and treatment group.Pyrro-lidinedithiocarbamate（PDTC） was given to the treatment group.Immunohistochemistry in situ detection was used to assry the expression of NF-kB after traumatic brain injury and the effect of PDTC on secondary brain injury.Results In the neurodegenerative area surrounding the lesion of injury group,a significative increasing of NF-kB was observed from 6h to 120h.In contrast,the expression of NF-kB was significantly downregulated by PDTC.Conclusion The study shows the activation of NF-kB may be the important mechanism of acute traumatic brain injury.And the NF-kB inhibitor may be a potential therapeutic method for the treatment of after traumatic brain injury.

关 键 词：创伤性脑损伤 NF-KB 继发性脑损伤 

分 类 号：R651.15[医药卫生—外科学]