基质金属蛋白酶-9基因多态性与颈动脉粥样硬化微栓子形成的相关性研究  

Relationship between polymorphism of matrix metalloproteinases-9 gene and the form of carotid atherosclerosis micro embolus

作　　者：沈焱[1] 白向东[2] 马瑞莲[2] 杜秦川[2] 张淑敏[2] 魏欣[2] 李玲[2] 高志嵩[2] 程继明[2] 邓长林[2] 王静[2] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏银川市第一人民医院神经内科,宁夏银川750001

出　　处：《宁夏医学杂志》2013年第3期207-210,I0001,共5页Ningxia Medical Journal

基　　金：宁夏自治区自然科学基金资助项目(NZ11218);宁夏自治区卫生厅重点科研计划(2011048)

摘　　要：目的研究颈动脉粥样硬化微栓子阳性是否与基质金属蛋白酶-9(MMP-9)基因启动子C-1562T的多态性有相关性,为脑血管疾病的早期预防、诊断及治疗提供帮助。方法对被确诊为缺血性脑血管病的患者行颈动脉超声检查,筛选出患颈动脉粥样硬化不稳定斑块的患者120例,再行彩色经颅多普勒超声(TCD)监测微栓子,将其划分为微栓子阳性组63例与微栓子阴性组57例,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对MMP-9基因启动子C-1562T位点进行基因分型,并随机抽取10%的样本对MMP-9基因启动子C-1562T的基因型进行直接测序证实。统计2组患者中C-1562T的基因型、等位基因频率,并分析MMP-9基因启动子C-1562T的多态性是否与微栓子阳性有相关性。结果微栓子阳性组CT型和TT型明显高于微栓子阴性组,2组比较差异有统计学意义(χ2=32.568,P<0.05);MMP-9的T等位基因是微栓子形成的危险因素(OR=10.000,P<0.05)。结论 MMP-9基因启动子C-1562T基因多态性与微栓子形成密切相关。Objective To investigate the relationship between polymorphism at promoter of C - 1562 T of matrix metalloprotein- ases - 9 （ MMP - 9） and positive of carotid atherosclerosis micro embolus, and to provid the help for cerebrovascular disease prevention, early diagnosis and treatment. Methods A diagnosis of carotid ultrasound ischemie cerebrovascular were done for disease patients, and they were screened with 120 patients who had unstable plaques with carotid atherosclerosis. Further, color Transcranial Doppler ultra- sound （TCD） were used to monitor micro embolus and divided into the positive of micro embolus patients group （n = 63 ） and the nega- tive of micro embolus patients group （ n = 57）. At the same time application polymerase chain reaction - restriction fragment length poly- morphism （PCR - RFLP） technology was applied to type gene sites of MMP -9 polymorphism, and extract 10% the random samples to confirmed genotype of promoter C -1562T of MMP-9. Finally, using the statistics to compare the two groups genotype and allele fre- quency, and analyzes the promoter C - 1562 T of MMP -9 the polymorphism of whether there was a correlation with micro embolus posi- tive. Results CTtype and TY type were significantly higher in micro embolus positive group than these in the control group. There was statistical significance in the two groups （X2 = 32.568, P = 0. 000,P 〈 0.05 ）. The T allele of MMP- 9 was a risk factor of the forma- tion of micro embolus （ OR = 10. 000, P 〈 0.05 ）. Conclusion MMP - 9 gene promoter C - 1562 T sites intimately relate to the forma- tion of micro embolus.

关 键 词：基质金属蛋白酶-9(MMP-9) 缺血性脑血管病 颈动脉粥样硬化不稳定斑块 微栓子. 

分 类 号：R743.3[医药卫生—神经病学与精神病学]