鸡、猪大肠杆菌ESBLs基因型检测及耐药性分析  

Genotype Detection of ESBLs and Antibiotic Resistance Analysis in E.coli Strains from Chickens and Pigs

作　　者：曲志娜[1] 张颖[1] 李玉清[1] 李梁梁[2] 王娟[1] 黄秀梅[1] 刘焕奇[2] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]青岛农业大学动物科技学院,山东青岛266109

出　　处：《中国农学通报》2013年第8期50-54,共5页Chinese Agricultural Science Bulletin

摘　　要：为检测不同动物源大肠杆菌中超广谱β-内酰胺酶(ESBLs)的基因型,探究ESBLs对大肠杆菌耐药性的影响,指导兽医临床有效防治大肠杆菌病。采用NCCLS标准方法和双纸片协同法,对102株大肠杆菌菌株进行抗菌药物敏感性测定和和ESBLs检测,设计合成4对引物,用PCR方法进行了ESBLs耐药质粒的DNA扩增和基因型分析。结果表明:以产ESBLs细菌的耐药质粒为模板扩增出了与预期片段大小相符的TEM、CTX-M型ESBLs产物,但均未扩增出SHV、OVA型ESBLs产物。鸡源、猪源产ESBLs菌株检出率分别为81%、6%。CTX-M、TEM型为山东莱西地区动物源产ESBLs大肠杆菌菌株的流行基因型,且鸡源产ESBLs大肠杆菌菌株分布广泛,应加强当地产酶耐药菌的监测和研究,以有效防治此类细菌引发的疾病。In order to detect genotype of extend spectrum β-lactamase （ESBLs） in E.coli isolates from different animal,and explore the influence of ESBLs to E.coli resistance,to instruct to prevent and cure Colibacillosis in veterinary clinic effectively.We detected antimicrobial susceptibility and ESBLs for 102 E.coli strains by NCCLS standard method and Double-disk Synergy Test,and designed and synthetized four pair of primers,studied ESBLs gene type with amplified and analyzed by PCR.The results showed that the ESBLs producing bacteria amplified by PCR with TEM-type and CTX-M-type down-object while SHV-type and OVA-type down-object did not.The detection rate of the ESBLs producing E.coli strains from chickens and pigs was 81% and 6% respectively.CTX-M-type and TEM-type were epidemic genotype of ESBLs in Shandong Laixi Region,and the ESBLs producing E.coli strains from chickens were widespread.We should reinforce monitoring and studying about the ESBLs producing drug-resistant bacteria in order to prevent and cure Colibacillosis in veterinary clinic effectively.

关 键 词：大肠杆菌 ESBLS 耐药性 基因型 

分 类 号：S859.1[农业科学—临床兽医学]