还原型谷胱甘肽对失血性休克型急性肺损伤相关炎性因子及抗氧化因子的调控及肺损伤的保护作用研究  

Protective Effects of Reduced Glutathione on SD Rats Hemorrhagic Shock Acute Lung Injury

作　　者：刘振威[1] 高风英 方阅[3] 

机构地区：[1]上海交通大学附属第一人民医院呼吸科,上海市200086 [2]上海市建工医院呼吸科 [3]上海交通大学附属第一人民医院药剂科

出　　处：《实用心脑肺血管病杂志》2013年第4期38-40,共3页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

摘　　要：目的研究还原型谷胱甘肽对SD大鼠失血性休克型急性肺损伤(ALI)相关炎性因子及抗氧化因子的调控作用和肺损伤保护作用。方法 SD大鼠24只,随机分为3组。A组为假手术组,只进行相应的手术操作;B组和C组制作失血性休克模型,造成ALI,然后在20min内复苏,C组复苏时应用还原型谷胱甘肽。测定动脉血浆中肿瘤坏死因子α(TNF-α)含量、支气管肺泡灌洗液(BALF)中巨噬细胞(PAM)和中性粒细胞(PMN)总数、BALF中蛋白含量和肺组织中超氧化物歧化酶(SOD)含量;计算肺组织含水率;光镜下观察肺组织形态学改变。结果与A组比较,复苏后B、C组TNF-α含量、BALF中的蛋白含量和PMN总数、肺组织含水率升高,SOD含量降低,差异均有统计学意义(P<0.05)。C组上述指标的变化程度均低于B组,差异有统计学意义(P<0.05)。光镜下见B组肺组织间质水肿、细胞浸润明显,与之相比C组肺组织以上改变明显减轻。结论还原型谷胱甘肽对SD大鼠的失血性休克型ALI具有保护作用。Objective To study the protective effects of Reduced Glutathione on SD rats hemorrhagic shock acute lung injury.Methods Twenty-four SD rats were divided into 3 groups randomly which named group A,B and C respectively.Group A was non-operation group,while hemorrhagic shock models were performed in both group B and group C,and resulted in ALI,then the shock rabbits were resuscitated in 20 min.The Reduced Glutathione was used in group C during resuscitation.The arterial blood was taken in different time to determine the plasma tumor necrosis factor-α（TNF-α）concentration.The amount of pulmonary alveolarmacrophages（PAM）and polymorphonuclear leukocyte（PMN）in bronchoalveolar lavage fluid（BALF）were counted atthe end of experiment.At the same time,the protein contents in BALF and superoxide dismutase（SOD）contents in lung tissue were measured,and the water ratio of lung tissue was calculated.The histological forms of lung were examined through the light microscope.Results Compared with group A,B after resuscitation,C group content of TNF-α,BALF protein content and total PMN,lung tissue water content was significantly increased,SOD content decreased significantly,the difference was statistically significant（P0.05）.Changes of C group were all lower than those of B group,the difference was statistically significant（P0.05）.Under light microscope,lung tissue in group B interstitial edema,infiltration significantly,compared with the above of lung tissue in C group was obviously alleviated the changes.Conclusion Reduced Glutathione has the protective effects on ALI which resulted from experimental hemorrhagic shock in SD rats.

关 键 词：还原型谷胱甘肽 SD大鼠 休克 出血性 呼吸窘迫综合征 成人 保护作用 

分 类 号：R-332[医药卫生]