慢性应激抑郁模型大鼠海马组织细胞色素C的表达及抗抑郁药物的干预作用  

Expression of Cytochrome C in Hippocampus of Chronic Stress Depression Rats and the Interference of Antidepressants

作　　者：孔令韬[1] 吴枫[1] 汤艳清[1] 

机构地区：[1]中国医科大学附属第一医院精神医学科,辽宁省沈阳市110001

出　　处：《中国全科医学》2013年第9期1008-1011,共4页Chinese General Practice

基　　金：国家自然科学基金资助项目(81101012;81071099);辽宁省博士科研启动基金(20111099)

摘　　要：目的观察慢性应激抑郁模型大鼠海马组织细胞色素C的表达及抗抑郁药物的干预作用。方法将雄性Wistar大鼠60只采用随机排列表法分为对照组、模型组、氟西汀组和噻萘普汀组,每组15只。模型制备期间对大鼠进行刺激前,氟西汀组大鼠采用氟西汀10.0 mg/kg灌胃,噻萘普汀组大鼠采用噻萘普汀50.0 mg/kg灌胃,对照组和模型组大鼠采用等体积的0.9%氯化钠溶液灌胃。采用蛋白印迹法测定各组大鼠海马组织细胞色素C的表达水平,以整合光密度值(IDV)表示;采用开场法测定各组大鼠模型制备前后5 min内的行为学观察指标,包括水平穿越格数、竖立次数、修饰次数,之后进行液体消耗实验,计算24 h内蔗糖水消耗百分比。结果对照组大鼠平均IDV值为(24 631±2 194),模型组为(38 963±5 384),氟西汀组为(27 220±2 892),噻萘普汀组为(33 204±4 174),4组大鼠细胞色素C表达水平比较,差异有统计学意义(F=33.343,P=0.000)。4组大鼠模型制备前水平穿越格数、竖立次数、修饰次数及蔗糖水消耗百分比比较,差异均无统计学意义(P>0.05);制备后比较,差异均有统计学意义(P<0.05)。结论慢性应激抑郁模型大鼠海马组织细胞色素C表达水平降低,抗抑郁药物可能通过抑制神经元内线粒体细胞色素C的释放以减少应激导致的神经元凋亡。Objective To investigate the expression of Cytochrome C in hippocampus of chronic stress depression rats and the interference of antidepressants. Methods 60 Wistar rats were randomly divided into control group, model group, fluoxe- fine group and tianeptine group with each group 15 rats. Before stimulation to the rats during model preparation, the fluoxetine group was given intragastric administration of 10. 0 mg/kg fluoxetine, the tianeptine group was given intragastrie administration of 50. 0 mg/kg tianeptine, and the control group and model group were both given intragastric administration of 0. 9% sodium chlo- ride solution with the same volume. The expression of Cytochrome C was detected by Western Blot method and was expressed by IDV. Ethology indexes （including horizontal squares crossed, erection times and modification times） five minutes before and af- ter model establishment were detected by open - field method, and experiment of fluid consumption was conducted to calculate the percentage of saechar - consumption within 24 h. Results The average IDV was （ 24 631±2 194 ） , （ 38 963±5 384 ）, （27 220±2 892） and （33 204± 4 174 ） respectively in control group, model group, fluoxetine group and tianeptine group, and the difference was statistically significant （F = 33. 343, P = 0. 000） . The horizontal squares crossed, erection times, mod- ification times and percentage of sacehar - consumption between the four groups showed no statistically significant differences be- fore model establishment （ P 〉 0. 05 ） , but showed statistically significant differences after model establishment （ P 〈 0. 05 ）. Conclusion The expression of Cytochrome C in hippoeampus of chronic stress depression rats decreases and can be reversed by some antidepressants through inhibiting the release of Cytochrome C of mitoehondria in neuron, thus reducing neuronal apoptosis caused by stress.

关 键 词：抑郁症 应激 海马 细胞色素C 氟西汀 噻萘普汀 

分 类 号：R749.42[医药卫生—神经病学与精神病学]