环境温度变化对大鼠肺组织病理损伤的观察  

Effect of Ambient Temperature Changes on Lung Tissue Injury in Rats

作　　者：傅珏[1] 曾慧红[2] 杨晓莉[3] 吴芳庚[4] 傅爱香[2] 朱清仙[2] 

机构地区：[1]南昌大学抚州医学分院病理教研室,江西抚州344000 [2]南昌大学医学院组织胚胎学教研室,南昌330006 [3]南昌大学抚州医学分院组织胚胎学教研室,江西抚州344000 [4]江西省肿瘤医院病理科,南昌330029

出　　处：《南昌大学学报（医学版）》2013年第1期5-7,25,共4页Journal of Nanchang University：Medical Sciences

基　　金："十一五"国家科技支撑计划(2008Bai68b01);江西省卫生厅科技计划(20112025)

摘　　要：目的探讨环境温度变化时大鼠肺组织的病理损伤及规律。方法将9～15周龄雄性SD大鼠84只按随机数字表法分为常温对照组(20℃,21只)和温度骤变组[骤升温至37℃组(21只)、骤降温至4℃组(21只)和骤降温至-12℃组(21只)],每组均观察1、4、8、12、24、48和168h7个时间点,每个时间点3只大鼠。分别于各时间点处死动物,切取左侧肺脏上叶,经4%多聚甲醛液固定24h、石蜡包埋和切片,常规HE染色观察肺组织病理学变化。结果温度骤变组大鼠肺组织均出现不同程度的损伤,主要表现在肺泡壁毛细血管扩张充血、出血、细支气管黏膜上皮坏死、炎细胞浸润等,在骤升温至37℃组最严重。各组出现损伤最严重的时间不同,骤升温至37℃组在12h损伤最严重,而骤降温至4℃组和骤降温至-12℃组均在24h损伤最明显。结论环境温度变化可引起肺组织损伤,出现肺泡壁毛细血管扩张充血、出血、细支气管黏膜上皮坏死、炎症细胞浸润等病理变化。Objective To investigate the lung tissue injury in rats following ambient tempera- ture changes. Methods Eighty-four male SD rats aged 9-15 weeks were randomized into fourgroups： normal temperature （20 ℃ group） , sudden rise in temperature to 37 ℃ （37 ℃ group）, sudden drop in temperature to 4 ℃ （4 ℃ group） and sudden drop in temperature to - 12 ℃（-12 ℃ group）,with 21 rats in each group. Three rats in each group were observed at 1, 4,8, 12,24,48 and 168 hours, respectively. Animals were sacrificed at different time points. The upperlobes of the left lung were collected, fixed in 4 % paraformaldehyde solution for 24 hours. Paraffinembedded tissue sections were used for HE staining to observe pulmonary histopathological than-ges. Results Different degrees of damage was found in lung tissues in rats undergoing ambient temperature changes, especially in group 37 ℃ group. Lesions mainly included capillarectasia,congestion, bleeding,bronchial mucosal epithelial necrosis and inflammatory cell infiltration. The most obvious damage was found at 12 hours in 37 ℃ group,at 24 hours in 4 ℃ group and at 24hours in -12 ℃ group. Conclusion Ambient temperature changes can cause lung tissue injury,including capillarectasia,congestion, haemorrhagia, bronchial mucosal epithelial necrosis and inflammatory cell infiltration.

关 键 词：环境温度变化 肺组织 病理损伤 动物 实验 大鼠 

分 类 号：R-332[医药卫生]