猪背最长肌与腰大肌microRNA表达谱分析  

Study on MicroRNA Transcriptome between Porcine Longissimus dorisMuscle and Psoas majorMuscle

作　　者：张小丽[1] 刘瀛锴[1] 李明洲[1] 蒋岸岸[1] 帅素容[1] 

机构地区：[1]四川农业大学动物遗传育种与繁殖研究所,四川雅安625014

出　　处：《四川农业大学学报》2013年第1期71-74,114,共5页Journal of Sichuan Agricultural University

基　　金：国家转基因生物新品种培育重大专项(2011ZX08006-003);现代农业产业技术体系建设专项基金项目(NYCYTX-009)

摘　　要：【目的】为研究microRNA在猪肌肉生长发育中的作用。【方法】采用Illumina高通量测序技术对猪背最长肌和腰大肌的microRNA转录组进行测定,并鉴定差异表达的microRNA。【结果】在猪背最长肌和腰大肌文库中分别产生了15.22M和17.52M的序列;两个文库共检测到的695个microRNA,其中有363个共表达、193个差异极显著(P<0.01)。【结论】说明microRNA在不同骨骼肌类型中存在差异表达。【Object】This experiment was conducted to investigate the effects of MicroRNAs in the development of skeletal muscle.【Method】Illumina Solexa sequencing technolog were applied to acquire the miRNA expression patterns between porcine longissimus doris muscle and psoas major muscle. 【Result】a total of 15.22M and 17.52M mappable sequences were expressed in longissimus doris muscle and psoas major muscle library respectively.Totally 695 unique miRNAs were observed, and 363 were co-expressed in two libraries, of which 193 were significantly differentially expressed between longissimus doris muscleand and psoas major muscle library （P〈 0.01）. 【Conclusion】All these differences indicated the essential roles of miRNAs in the phenotypic variations of different muscle fiber types.

关 键 词：猪 MICRORNA 背最长肌 腰大肌 高通量测序 

分 类 号：S828.8[农业科学—畜牧学]