抑制MMP9表达对黑色素瘤细胞生物学的影响  

The influence of MMP9 expression suppression on the biological behavior of melanoma cells

作　　者：张国安[1] 韩莎[2] 周健[3] 侯森[1,4] 王学春[5] 崔文[1] 

机构地区：[1]济宁医学院基础学院 [2]济宁医学院中心实验室 [3]济宁医学院司法鉴定中心,山东济宁272067 [4]山东省医学科学院基础所,山东济南250062 [5]泰山医学院基础学院,山东泰安271000

出　　处：《济宁医学院学报》2013年第1期18-21,共4页Journal of Jining Medical University

基　　金：山东省教育厅计划项目(J06L06)

摘　　要：目的探讨抑制基质金属蛋白酶9(MMP9)对高转移黑色素瘤细胞系WM451生物学的影响。方法利用小干扰RNA(siRNA)沉默MMP9的表达,western blot检测MMP9表达降低情况,利用CCK-8、黏附实验、划痕实验对细胞生物学改变进行观测。结果 siRNA降低了MMP9的表达,伴随MMP9表达的降低,WM451增殖减缓,黏附能力和运动能力下降。结论黑色素肿瘤细胞系的侵袭潜能与其产生MMP9的能力密切相关。Objective To investigate the influence of MMP9 expression suppression on the biological behavior of melanoma cells. Methods We used siRNA to suppress the expression of MMPg,and then western blot was used to confirm the expression of MMPg. After that,CCK-8 assay,adhesion assay,and wound healing assay were used to measure the biological behavior of WM451. Results MMP9 expression was decreased by siRNA. The ability of growth,adhesion and invasion were also suppressed with the clown-regulation of MMPg. Conclusion The growth and invasive potential of this melanoma cell line are correlated with their expression of MMPg.

关 键 词：黑色素瘤 MMP9 肿瘤转移 

分 类 号：R739.5[医药卫生—肿瘤]