AAV2/9介导的PD-L1保护移植物的实验研究  

EXPERIMENTAL STUDY ON ADENO-ASSOCIATED VIRUS 2/9 MEDIATED PD-L1 GENE TRANSFER TO THE GRAFT PROTECTING ALLO-REJECTION

作　　者：罗汕[1] 蒋小峰[1] 杨学伟[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260

出　　处：《现代医院》2013年第3期10-12,共3页Modern Hospitals

摘　　要：目的探讨腺相关病毒介导的PD-L1在移植排斥反应中的作用。方法用含AAV2/9-PD-L1或LacZ的UW液灌注供体心脏后,移植到受体大鼠体内,观察移植物的转染效率和存活时间。结果AAV2/9可以高效地介导外源性基因转染移植物,AAV2/9-PD-L1转染移植物后虽能延长移植物的存活时间,但和对照组比较无统计学意义。结论 AAV2/9是一种高效安全的病毒载体,PD-L1局部表达抑制排斥反应的机制还需进一步研究。Objective To investigated the effect of Adeno - associated viral vectors 2/9 （AAV2/9） mediated program death ligand 1 （ PD - L1 ） gene transfer to myocardium on cardiac allograft survival in a rat heart transplantation model. Methods Animals were divided into three groups ： GroupPD - LI ： 0. 3 ml UW solution containing AAV2/9 -PD -L1 （5 x 1012 vector genome,vg） was infused into Brown Norway （BN）rat hearts prior to be transplanted into Lewis rats. In control group, null AAV2/9 viral vectors and UW solution were applied. To evaluate the gene transfection efficiency, additional group of AAV2/9 - LacZ were used in iso - transplantation group. Results Efficient transfection was demonstrated by application of AAV2/9 - LacZ in iso - graft. Successful transfection of AAV2/9 - PD - LI prolonged the allograft survival but without statistical significance. Conclusion Gene transfer of PD - L1 to graft could protect the allograft from the allo - rejection. The mechanism should be further studied.

关 键 词：基因转染 腺相关病毒 心肌 

分 类 号：R394[医药卫生—医学遗传学]