桂西地区原发性无精子症和严重少精子症患者Y染色体无精子因子检测分析  

Analysis of measure of Y chromosome azoospermia factor microdeletions in patients with idiopathic azoospermia and severe oligozoospermia in west Guangxi area

作　　者：潘云[1] 卢冬[1] 潘国刚[1] 李北林[1] 

机构地区：[1]右江民族医学院附属医院检验科,广西百色533000

出　　处：《检验医学与临床》2013年第6期645-646,共2页Laboratory Medicine and Clinic

基　　金：广西教育厅资助项目(200710LX125)

摘　　要：目的探讨桂西地区原发性无精子症和严重少精子症患者Y染色体无精子因子(AZF)微缺失状况。方法采用多重聚合酶链反应技术对桂西地区56例原发无精子症、78例原发严重少精症患者及40例正常生育男性进行4个区域15个位点微缺失分析。结果 40例正常生育男性未发现Y染色体AZF微缺失,134例生精障碍患者中发现AZF微缺失14例,总缺失率为10.5%。生精障碍组与正常生育组比较Y染色体AZF微缺失率差异具有统计学意义(P<0.01)。结论 Y染色体AZF微缺失是男性原发无精、少精子症的重要原因之一,AZF微缺失检测对男性不育症患者进行遗传学诊断与筛查有一定意义。Objective To investigate the microdeletion situation of Y chromosome azoospermia factor（AZF） in the patients with idiopathic azoospermia and severe oligozoospermia in the. west Guangxi area. Methods Multiplex PCR was used to detect Y chromosome microdeletion in 15 STS in 56 cases of idiopathic azoospermia,78 cases of se- vere idiopathic oligozoospermia and 40 healthy male controls. Results No Y chromosome AZF microdeletion was found in 40 controls. Y chromosome AZF microdeletion was found in 14 cases among 134 azoospermia patients,the total prevalence rate of the total microdeletion was 10.5 %. Difference of microdeletion rate between two groups was statistically significant（P〈0.01）. Conclusion Y chromosome AZF microdeletions is one of important reasons in idi- opathic azoospermia and oligozoospermia. The detection of AZF factor may play an important role in the genetic diag- nosis and screening among male infertility patients.

关 键 词：无精子因子 微缺失 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学]