我国3个主产棉区转Bt/CpTI基因棉田根际土壤中Cry1Ab蛋白的降解动态  

Dynamics of Cry1Ab Protein Degradation in Rhizospheric Soil of Bt /CpTI-Transgenetic Cotton Fields in Three Major Cotton Production Areas of China

作　　者：孟军[1] 方志翔[1] 刘标[1] 

机构地区：[1]环境保护部南京环境科学研究所国家环境保护生物安全重点实验室,江苏南京210042

出　　处：《生态与农村环境学报》2013年第2期260-264,共5页Journal of Ecology and Rural Environment

基　　金：国家转基因生物新品种培育重大专项(2011ZX08012-005;2008ZX08011-002)

摘　　要：于2009—2010年棉花种植期,在长江流域、黄河流域和西北内陆3个主产棉区分别选择代表地———江西九江、河南新乡和新疆喀什,采集长期种植转Bt/CpTI棉SGK321及其亲本石远321棉(对照)的棉田根际土壤样品,采用酶联免疫吸附法对根际土壤中Cry1Ab蛋白含量进行残留动态监测。结果显示,在各产棉区棉花蕾期、花期和铃期,转Bt/CpTI棉田根际土壤Cry1Ab蛋白含量大多显著高于对照棉田;而在棉花成熟期,转Bt/CpTI棉田根际土壤Cry1Ab蛋白含量与对照棉田大多无显著差异。长期种植转Bt/CpTI基因棉花不会造成Cry1Ab蛋白在棉花根际土壤中的累积。During the cotton growing periods of 2009 - 2010, soil samples were collected from the rhizospheres of cottons of SGK321 （Bt/CpTI-transgenic cotton） and Shiyuan 321, seed parent of the former, as control, in cotton fields that had long been cultivated with that respective variety of cotton, in Jiujiang of Jiangxi, Xinxiang of Henan and Kashi of Xinjiang, three major cotton production areas of China, in the Yangtze River Valley, Yellow River Valley and the inland of North-west China, respectively, for monitoring of dynamics of Cryl Ab protein residuals in the rhizospheres, using the enzyme-linked immunosorbent assay （ELISA）. Results show that CrylAb protein content was significantly higher in soil samples collected from SGK321 cotton fields at the budding, flowering and holl bearing stages than in those from Shiyuan 321 cotton fields, but no significant difference was found between the two collected at the maturing stage. The findings indicate that long-term cultivation of Bt/CpTI-transgenic cotton would not lead to accumulation of Cryl Ab protein in the cotton rhizospheric soil.

关 键 词：转Bt CpTI基因棉花 根际土壤 Cry1Ab蛋白 降解动态 

分 类 号：X171.1[环境科学与工程—环境科学]