Beclin1和LC3在乳腺导管癌组织中的表达  

Expression of Beclin1 and LC3 in the tissue of invasive ductal carcinoma

作　　者：周国华[1,2] 姚维深[1,2] 张旭[3] 

机构地区：[1]南海人民医院 [2]南方医科大学附属南海医院胸外科,广东佛山528200 [3]中山大学肿瘤防治中心胸外科,广东广州510060

出　　处：《海南医学》2013年第5期630-632,共3页Hainan Medical Journal

基　　金：佛山市医学类科技攻关项目(编号:201208135)

摘　　要：目的探讨Beclinl和LC3在乳腺浸润性导管癌中的表达及两者的相关性。方法免疫组织化学EnVision法检测50例乳腺浸润性导管癌及相应癌旁组织的Beclin1和LC3表达,分析其临床意义。结果乳腺癌组织Beclin1阳性表达率[44%(22/50)]明显低于癌旁组织[100%(50/50)],LC3阳性表达率[40%(20/50)]明显低于癌旁组织[92%(46/50)];Beclin1和LC3表达均与患者病理分级、临床分期和淋巴结转移情况有关(P<0.05),而与患者年龄、绝经状况、乳头侵犯及患者ER、PR依赖性无关(P>0.05);Pearson相关性分析显示两者在乳腺癌中的表达无相关性。结论乳腺导管癌组织中LC3和Beclin1阳性表达率低于癌旁组织,两者可能均与乳腺癌的发生、发展相关。Objective To investigate the expression of Beclinl and LC3 in the tissue of invasive ductal carci-noma （IDC） and to evaluate the relationship of them. Methods Immunohistochemical method was used to determine the expression of Beclinl and LC3 protein in 50 patients of IDC and nearby mucosa of the carcinoma. The relation-ship between the gene expression and clinicopathological features was analyzed. Results The positive expression rate of Beclinl in IDC （44%, 22/50） was significantly lower than that in nearby mucosa of the carcinoma （100%, 50/50）. The positive expression rate of LC3 in IDC （40%, 20/50） was significantly lower than that in nearby mucosa of the car-cinoma （92%, 46/50）. The expression of Beclinl and LC3 were correlated with the clinicopathological features, tissue types and neck lymph node metastasis in IDC （P〈0.05）, but showed no correlation with ages, menopausal status, nip- ple infringement status, and the patients＇ ER and PR dependence （P〉0.05）. The expression of Beclinl was not correlat- ed with the expression of LC3. Conclusion Expression of Beclinl and LC3 is down-regulated in IDC tissues, which might be related to the tumorigenesis and development oflDC.

关 键 词：乳腺癌 乳腺浸润性导管癌 BECLIN1 LC3 

分 类 号：R737.9[医药卫生—肿瘤]