分光光度计法测定磷脂酶A1酶活条件优化  

Optimization of Colorimetric Method for Phospholipase A1 Activity Assay

作　　者：苏燕南[1] 薛正莲[1] 马琦亚[1] 王洲[1] 赵世光[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《重庆理工大学学报（自然科学）》2013年第3期22-27,共6页Journal of Chongqing University of Technology：Natural Science

基　　金：安徽省自然科学基金资助项目(11040606M81);安徽省高校自然科学基金资助项目(KJ2009A168)

摘　　要：优化了分光光度计法测定Lecitase Ultra的磷脂酶A1活力的相关参数。根据单因素实验结果选择底物浓度、酶稀释倍数、pH值3个因素进行正交实验,经分析得到酶活测定的最佳条件为:酶稀释倍数为1 250倍,缓冲体系pH值为6.5,离子强度为50 mM,底物浓度为6%,终止酶反应方式为加入0.4M TCA溶液。优化后酶活最高值为4 876.9 U/mL,较原工艺提高了18.42%。The relevant parameters of colorimetric method for phospholipase A1 activity assay was optimized.Three factors,substrate concentration,enzyme dilution factor and pH,were chose for orthogonal experiment based on single factor experiment.The optimal conditions for enzyme assay were as follows: enzyme solution diluted for 1250-fold,pH 6.5,ionic strength of 50 mM,substrate concentration of 6%,adding 0.4M TCA for enzyme reaction termination.The activity reached a maximum value of 4876.9U/mL,which was increased 18.42% compared to the original process.

关 键 词：磷脂酶A1 LECITASE ULTRA 分光光度计法 正交实验 

分 类 号：TS201.25[轻工技术与工程—食品科学]