结直肠癌线粒体脱氧核糖核酸拷贝数与患者预后的关系  

Relationship between copy.number of mitochondrial DNA and prognosis of patients in coiorectal cancer

作　　者：崔海宏[1] 黄平[1] 张鋆歆[2] 张振华[1] 徐伟[1] 王小鹏[1] 韩英[3] 

机构地区：[1]中国人民解放军第三0五医院消化科,北京100017 [2]中国人民解放军第三0五医院病理科,北京100017 [3]中国人民解放军北京军区总医院消化科,北京100017

出　　处：《中华消化杂志》2013年第3期180-183,共4页Chinese Journal of Digestion

摘　　要：目的探讨结直肠癌（CRC）线粒体DNA（mtDNA）拷贝数异常与患者预后的关系。方法选取60份CRC及相应癌旁组织标本，分别提取基因组DNA。对线粒体NDl基因进行荧光定量PCR,计算mtDNA拷贝数，采用￡检验进行统计分析。Kaplan-Meier生存分析判断患者的预后。结果CRC组织的mtDNA平均拷贝数／细胞数为108.60±20.11，而相应的癌旁组织为153.68±25.72，前者显著低于后者（t=10.69，P〈0.01）。淋巴结转移阳性者mtDNA低拷贝率高于阴性者（X^2=4.022，P〈0.05），但与性别、年龄、病理分型、TNM分期无明显相关性。mtDNA高拷贝组生存率高于低拷贝组，但差异无统计学意义（P〉0.05）。结论CRC的mtDNA拷贝数明显低于癌旁组织，但拷贝数的改变与患者的预后无关。Objective To explore the relationship between abnormal copy number of mitochondrial DNA （mtDNA） and prognosis of the patients in colorectal cancer （CRC）. Methods A total of 60 cases of CRC and corresponding adjacent noncancerous tissue specimens were selected. Genomic DNA was extracted. The mitochondrial ND1 gene was detected by quantitative real-time polymerase chain reaction and 13-actin was set as internal control. And then the copy number of mtDNA was caculated and analyzed by t-test. The prognosis of patients was determined by Kaplan-Meier survival analysis. Results The mean mtDNA copy number of CRC tissue was 108.60±20.11, while that of the corresponding adjacent noncancerous tissues was 153.68±25.72, the former was significantly lower than that of the latter （t=10.69, P〈0.01）. The rate of low mtDNA copy number in case with positive lymph nodes metastasis was higher than that in cases with negative lymph nodes metastasis （x^2= 4.022, P〈0.05）, however there were no obvious correlation with gender, age, pathological type, TNM stage. The survival rate of high mtDNA copy number group was higher than that of low mtDNA copy number group, however there was no statistical significance （P〉0.05）. Conclusions mtDNA copy number of CRC was significantly lower than that of the adjacent noncancerous tissues. However the change of copy number was not correlated to the prognosis.

关 键 词：结直肠肿瘤 DNA 线粒体 聚合酶链反应 淋巴转移 预后 

分 类 号：R735.3[医药卫生—肿瘤]