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作 者:陈志坚[1] 潘鲁燕[1] 张慧[1] 张惠娟[1] 李加友[1]
机构地区:[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001
出 处:《安徽农业科学》2013年第5期2058-2060,共3页Journal of Anhui Agricultural Sciences
基 金:江苏省自主创新项目CX(12)1002;生物工程专业教学团队项目
摘 要:研究土壤中污染物含量与微生物多样性之间的关系,评估污染物对土壤生态的影响,实现土地资源的可持续发展。以邻氨基苯甲醚污染土壤为研究对象,利用氯仿熏蒸法测定土壤中生物量(Bc),并采用培养和形态分类法测定可培养土壤微生物种类,建立邻氨基苯甲醚含量与土样中微生物多样性关系的数学模型。研究结果表明,Bc与邻氨基苯甲醚含量呈现负相关,并且它对不同种类微生物的影响不同,其中对真菌有显著抑制作用,而所检测土样中不能检测到可培养放线菌。因此,通过考察土壤中微生物多样性的变化,可以评估土壤受邻氨基苯甲醚影响的程度。The relationship between concentration of pollutant and diversity of microorganisms was investigated to estimate the ecological impact of pollutant and to promote the sustainable development of soil.The soil samples were polluted with 2-Methoxy-Benzenamine.The soil microbial biomass carbon(Bc) was detected with chloroform fumigation and the cultivable microorganisms were identified with morphology methods.The mathematic model about the relationship between the pollutant,2-Methoxy-Benzenamine,and the diversity of microorganism was established.The results showed that the Bc and 2-Methoxy-Benzenamine content was negatively related to the present,and various microorganisms were affected by the pollutant differently.The fungi was inhabited remarkably and the actinomycete was not detected in the culture.Based on the above study,we could estimate the influence of pollutant on the soil with the change of microbiologic diversity.
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