健脾益智胶囊对大脑中动脉栓塞大鼠海马神经干细胞增殖及Notch1、Jagged1表达的影响  

Effects of Jianpi Yizhi Capsule on proliferation of NSCs and expression of Notch1 and Jagged1 in the hippocampus of MCAO rats

作　　者：冯珂[1] 纪立金[2] 

机构地区：[1]山东中医药大学,济南250355 [2]福建中医药大学,福州350122

出　　处：《中华中医药杂志》2013年第4期921-924,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30973708)~~

摘　　要：目的:探讨健脾益智胶囊对大脑中动脉栓塞(MCAO)大鼠海马神经干细胞(NSCs)增殖及Notch1、Jagged1基因与蛋白表达的影响。方法:建立MCAO大鼠模型,随机分为正常组、假手术组、模型组、西药组、中药组。采用免疫组化、Western Blotting、Real-Time RT-PCR法于术后7d、14d、28d观察海马NSCs增殖,Notch1、Jagged1蛋白及mRNA表达情况。结果:术后7d中药组NSCs、Notch1、Jagged1蛋白及mRNA表达显著高于模型组、西药组(P<0.05)。术后28d中药组NSCs表达显著高于模型组、西药组(P<0.05);Notch1蛋白及mRNA低于正常、假手术组,但高于模型组、西药组(P<0.05)。结论:健脾益智胶囊可促进BrdU阳性细胞数量增加,提高Notch1、Jagged1蛋白及基因的表达,提示健脾益智胶囊能可能通过激活Notch通路以促进缺血后神经干细胞增殖分化。Objective： To detect the effects of Jianpi Yizhi Capsule on proliferation of NSCs,protein and mRNA expression levels of Notch1 and Jagged1 in the hippocampus of MCAO rats.Methods： 120 SD rats were randomly divided into 5 equal groups： normal control group,sham operation group,middle cerebral artery occlusion（MCAO） group,Nimodipine group,Jianpi Yizhi group.7,14 and 28 days after the operation,the proliferation of NSCs,protein and mRNA levels of Notch1 and Jagged1 in hippocampus were assayed respectively by immunohistochemistry,Western Blotting,RT-PCR methods.Results： The NSCs,protein and mRNA expression levels of Notch1 and Jagged1 on the 7th day of the Jianpi Yizhi group were significantly higher than MCAO and Nimodipine group（P0.05）.On the 28th day,the proliferation of NSCs of Jianpi Yizhi group was significantly higher than MCAO group and Nimodipine group（P0.05）;the protein and mRNA levels of Notch1 and Jagged1of Jianpi Yizhi group was lower than normal control group,sham operation group,but higher than MCAO group and Nimodipine group（P0.05）.Conclusion： Jianpi Yizhi Capsule can increase the BrdU positive cells,protein and gnne expression of Notch1 and Jaggedl,which suggests that the Jianpi Yizhi Capsule may active the Notch pathway to regulate the the proliferation and differentiation of neural stem cells in the dentate gyrus of hippocampus after ischemia.

关 键 词：健脾益智胶囊 大脑中动脉栓塞 神经干细胞 Notch信号通路 NOTCH1 JAGGED1 

分 类 号：R285.5[医药卫生—中药学]