HB-EGF蛋白在胃癌组织和人胃癌细胞系中的表达  

Expression of HB-EGF protein in tissues and cell lines of human gastric cancer

作　　者：王国荣[1] 王小强[1] 车向明[2] 邱健[1] 郑焱[3] 刘瑞廷[1] 何文宪[1] 潘承恩[2] 

机构地区：[1]陕西省人民医院普外一科,陕西西安710068 [2]西安交通大学医学院第一附属医院普通外科,陕西西安710061 [3]西安交通大学医学院第二附属医院皮肤科,陕西西安710004

出　　处：《现代肿瘤医学》2013年第4期699-702,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81172363);陕西省科学技术研究发展计划资助项目[编号:2005k09-G3(3)]

摘　　要：目的:研究HB-EGF蛋白在胃癌组织和多个胃癌细胞系中的表达及其与胃癌发生的相关性。方法:免疫印迹和免疫荧光法检测新鲜胃癌组织和不同胃癌细胞系中HB-EGF蛋白的表达。结果:在癌旁正常胃黏膜中,HB-EGF蛋白表达较低,而在胃癌组织中,蛋白表达明显升高(P<0.05);在正常胃黏膜上皮细胞系GES-1中,未检出HB-EGF蛋白的表达,而在4种人胃癌细胞系中,HB-EGF蛋白表达均为阳性(P<0.01);其中SGC7901和MKN45中的表达强于BGC823和MKN28中的表达。结论:HB-EGF蛋白在胃癌组织和多种胃癌细胞系高表达,其表达水平升高可能与胃癌的发生和发展相关。Objective：To investigate the expression of HB -EGF in fresh gastric cancer tissues and different cell lines and its correlation with gastric cancer development. Methods： Expression of HB - EGF protein in gastric cancer tissues and cell lines were detected by western blot assays;Location of HB - EGF protein in gastric cancer cell lines were detected by Immunofluorescence staining. Results： In normal gastric tissues, the expression of HB - EGF was weak, however, was upregulated in gastric cancer tissues （P 〈 0.05） ;In human gastric cancer epithelial cell line GES - 1, none of HB - EGF signal was detected, in contrast, the expression of HB - EGF was high in all of four gastric cancer cell lines （ P 〈 0. 01 ） ; Furthermore, the expressions of SGC7901 and MKN45 were higher than that of the expression of BGC823 and MKN28. Conclusion： HB - EGF protein is highly expressed in gastric cancer and different gastric cancer cell hnes, and its elevated expression levels may implicate in the pathogenesis and development of gastric carcinoma.

关 键 词：胃癌 HB—EGF 免疫印迹 免疫荧光 

分 类 号：R730.23[医药卫生—肿瘤] R735.2[医药卫生—临床医学]