多靶标检测平台分析肺腺癌患者不同分化程度中ERCC1、TYMS、RRM1、TUBB3基因表达及意义  

By using liquidchip platform to detect mRNA expression of ERCC1,TYMS,RRM1 and TUBB3 in lung adenocarcinoma with various degrees of differentiation

作　　者：伍青[1] 朱彦君[1] 徐彪[2] 郭延林[1] 吴科[1] 阳晓波 

机构地区：[1]空军总医院胸外科,北京100142 [2]安徽医科大学,安徽合肥230001 [3]益善生物技术股份有限公司,广东广州510663

出　　处：《现代肿瘤医学》2013年第4期764-767,共4页Journal of Modern Oncology

基　　金：广东省中国科学院全面战略合作项目(编号:2011A090100024)

摘　　要：目的:通过一种mRNA表达水平多指标检测技术分析ERCC1、TYMS、TUBB3、RRM1在非小细胞肺腺癌(NSCLS)患者人群中的表达,探索基因表达与腺癌病理分化程度之间的相关性,并探讨其临床意义,为个体化用药临床指导提供基础。方法:应用分支DNA-液相芯片基因检测技术检测30例肺腺癌患者上述基因的mRNA水平表达,使用SPSS软件统计分析基因表达与腺癌分化程度之间的相关性。结果:ERCC1表达与腺癌分化程度有显著相关性(P<0.05),除此之外,其它靶标与腺癌分化程度之间并无显著相关性(P>0.05)。结论:ERCC1表达可能与腺癌分化程度之间具有相关性,腺癌中分化的患者ERCC1平均表达水平较低,低分化的患者ERCC1平均表达水平较高;同时,当ERCC1低表达时(24.1%),其他三个指标同时低表达的比例更低(分别为10.3%、6.9%、6.9%),因此确定用药方案之前,参考病理特征及靶标检测还是非常必要的。Objective： To explore the correlation between gene expression and degree of differentiation of lung adenocarcinoma of ERCC1 ,TYMS,TUBB3 ,RRM1 mRNA expression levels in non - small cell lung cancer patient by an innovative platform. Methods： Branched DNA liquid chip genetic was used to detect the gene expression of mRNA levels in 30 cases of lung adenocarcinoma patients. Results：ERCC1 expression related to the degree of addifferentiation （P 〈0. 05 ）. Conclusion：It was necessary to choose suitable regimen refer to genes and pathological characteristics. In this study,low ERCC1 expression level was easily seen in patients with moderate differentiation of adenoeareinoma. When low ERCC1 expression （24.1% ）was seen in lung adenocareinoma patients, at the same time, the proportion of the low expression level was more lower in the other three index （ TYMS 10.3% , RRM1 6.9% , TUBB3 6.9% respectively）.

关 键 词：ERCC1 TYMS RRM1 TUBB3 液相芯片 NSCLC 

分 类 号：R734.2[医药卫生—肿瘤]