RNA沉默环氧合酶2表达对人骨肉瘤细胞SaOS2的影响  

RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth and migration of SaOS2 human osteosarcoma cells

作　　者：刘胄[1] 吴兴洲[1] 宋锐[1] 宗训付 汝磊 李凯[2] 赵庆华[2] 

机构地区：[1]阜阳市肿瘤医院骨一科,安徽236000 [2]上海交通大学附属第一人民医院骨科

出　　处：《中华医学杂志》2013年第13期1028-1031,共4页National Medical Journal of China

摘　　要：目的观察沉默环氧合酶2（COX-2）基因对人骨肉瘤细胞SaOS2生长及转移的影响。方法利用慢病毒介导的RNA干扰技术沉默人骨肉瘤细胞SaOS2中内源性COX-2基因的表达，应用MTF和迁移实验等分别检测COX-2沉默对SaOS2增殖能力、迁移能力的影响；应用实时聚合酶链反应和免疫印迹检测COX-2、血管内皮生长因子（VEGF）、表皮生长因子（EGF）、成纤维细胞生长因子（bFGF）的mRNA和蛋白表达水平。结果转染LV-COX-2siRNA-1沉默COX-2后，SaOS2细胞生长受到了明显的抑制，直接降低了SaOS2细胞的迁移率；并能显著抑制VEGFA，EGF和bFGF的表达，而VEGFB和VEGFC的含量没有明显变化。结论RNA沉默人骨肉瘤中COX-2的表达能显著抑制SaOS2细胞的生长，降低其转移能力，并能下调VEGF、EGF和bFGF的mRNA和蛋白质的表达水平。Objective To explore the effects of a knockdown of cyclooxygenase 2 upon the growth and migration of SaOS2 cells. Methods We employed lentivirus mediated-RNA interference technology to knockdown the endogenous expression of gene COX-2 in human osteosarcoma cells （ SaOS： ） and analyzed their phenotypical changes. The effects of COX-2 silencing on the proliferation, cell cycle and migration of SaOS2 cells were assessed by thiazoyl blue tetrazolium bromide, flow eytometry and migration assays respectively. COX-2, vascular endothelial growth factor （VEGF）, epidermal growth factor （EGF）, basic fibroblast growth factor （bFGF） mRNA and protein expression were detected by reverse transcription- polymerase chain reaction （RT-PCR） and Western blot. Results A decreased expression of COX-2 in human osteosarcoma cells significantly inhibited the growth and decreased the migration ability of SaOS2 cells. Furthermore, it also reduced VEGF, EGF and bFGF mRNA and protein expression. Conclusion The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosareoma.

关 键 词：骨肉瘤 环氧合酶 干扰 

分 类 号：R738.1[医药卫生—肿瘤]