糖尿病对内皮祖细胞移植促血管新生作用的影响  

Does diabetes mellitus influence angiogenesis-promoting effects of bone marrow-derived endothelial progenitor cell transplantation?

作　　者：周晨光[1] 宫经新[2] 马跃[2] 周亚茹[3] 栾锋[4] 杨彦[2] 孟建波[2] 

机构地区：[1]河北工程大学附属医院血液内科,河北省邯郸市056000 [2]河北医科大学第三医院血液内科,河北省石家庄市050051 [3]河北医科大学第三医院内分泌科,河北省石家庄市050051 [4]河北医科大学第三医院五官科,河北省石家庄市050051

出　　处：《中国组织工程研究》2013年第6期1044-1048,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:内皮祖细胞为血管新生的前体细胞,通过促血管新生作用治疗糖尿病血管病变有着良好的前景。目的:探讨糖尿病对内皮祖细胞移植治疗缺血性疾病过程中促血管新生作用的影响。方法:制备糖尿病大鼠模型,提取糖尿病和正常大鼠供体骨髓单个核细胞体外定向培养为内皮祖细胞。同时建立糖尿病及正常大鼠下肢缺血模型,并于缺血病变部位局部移植糖尿病或正常大鼠内皮祖细胞或PBS进行对照。移植后定期应用ELISA方法检测病变部位血管内皮生长因子含量,应用免疫组化方法计数病变部位微血管密度。结果与结论:①受体相同,移植物不同时:移植糖尿病大鼠来源或正常大鼠来源内皮组细胞后,下肢缺血组织中血管内皮生长因子水平及微血管密度比较,差异无显著性意义(P>0.05)。②移植物相同,受体不同时:正常大鼠移植内皮祖细胞后下肢缺血组织中血管内皮细胞生长因子含量和微血管密度均高于糖尿病组。说明在体外定向培养和病变部位局部注射条件下,糖尿病对骨髓来源内皮祖细胞移植促血管新生作用无明显影响,而对血管新生所处的病变部位微环境有明显影响。BACKGROUND： As an angiogenic precursor cells, endothelial progenitor cells have a good prospect in the treatment of diabetic vascular disease by promotion of angiogenesis. OBJECTIVE： To investigate the effects of diabetes mellitus on the promotion of angiogenesis in the ischemic lower limb by transplantation of bone marrow-derived endothelial progenitor cells. METHODS： Rat models of diabetes mellitus were developed. Bone marrow mononuclear cells from the diabetes mellitus and normal rats were extracted and in vitro cultured towards endothelial progenitor cells. At the same time, diabetes mellitus and normal rat models of ischemic lower limb were established and received local transplantation of diabetes mellitus or normal rat endothelial progenitor cells or PBS into the ischemic region. After transplantation, vascular endothelial growth factor content and microvessel density in the lesion region were determined by ELISA and immunohistochemistry, respectively. RESULTS AND CONCLUSION： （1） When the recipients were the same, there were no significant differences in vascular endothelial growth factor content and microvessel density in the ischemic tissue between different donors after transplantation of endothelial progenitor cells from diabetes mellitus or normal rats （P 〉0.05）. （2） When the donors were the same, vascular endothelial growth factor content and microvesset density in the ischemic tissue were significantly greater in the normal rats than those in the diabetes mellitus rats. These findings suggest that diabetes meltitus exhibits no effects on the promotion of angiogenesis by local transplantation of the in vitro cultured endothelial progenitor cells but it greatly impacts the microenvironment of the lesion regions where angiogenesis occurs.

关 键 词：干细胞 干细胞移植 内皮祖细胞 移植 糖尿病 下肢缺血 血管新生 血管内皮细胞生长因子 微 血管密度 

分 类 号：R394.2[医药卫生—医学遗传学]