三氧化二砷／锰锌铁氧体复合纳米粒体外治疗食管癌  

Nanosized As_2 O_3 /Mn _0.5 Zn _0.5 Fe_2O_4 complex treats esophageal cancer in vitro

作　　者：路新丽[1,2] 王以薇[1] 张东生[2] 

机构地区：[1]江苏建康职业学院,江苏省南京市210029 [2]东南大学医学院,江苏省南京市210009

出　　处：《中国组织工程研究》2013年第8期1361-1361,共1页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81171452);江苏省卫生职业技术教育课题项目(J201007);江苏省高等学校大学生实践创新训练计划项目(979).

摘　　要：背景:与传统的热疗方法相比,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒可以同时发挥As2O3的细胞毒性作用和磁感应加热的联合定向治疗作用,效果优于单一治疗。目的:制备As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,观察其对食管癌Eca109细胞增殖的抑制作用。方法:采用浸渍法制备As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,含砷量0.012%,以透射电镜、能谱仪、原子分光光度仪对其进行表征。向两块培养板的食管癌Eca109细胞中分别加入DMEM培养液(阴性对照)、Mn0.5Zn0.5Fe2O4纳米材料、含As2O3终浓度5μmol/L的As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒、游离As2O3(终浓度5μmol/L),其中一块培养板进行磁流体热疗,另一块培养板正常培养。结果与结论:As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒近似球形,As2O3成功浸渍在Mn0.5Zn0.5Fe2O4纳米材料表面,砷含量在0.012%-0.066%之间。当As2O3浓度为5μmol/L时,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒组细胞增殖率明显低于阴性对照组和Mn0.5Zn0.5Fe2O4纳米材料组(P<0.05);而在磁流体热疗中,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒组细胞增殖率明显低于游离As2O3组或Mn0.5Zn0.5Fe2O4组(P<0.05);在凋亡率检测中,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒联合磁流体热疗组细胞凋亡率明显高于Mn0.5Zn0.5Fe2O4纳米材料联合磁流体热疗组或游离As2O3组(P<0.05)。表明As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒联合磁流体热疗可显著抑制食管癌细胞增殖。BACKGROUND： Compared with traditional hyperthermia treatment, As2O3/Mn0.5Zn0.5Fe2O4 complex may have both the cytotoxicity effects and the targeted heating effects resulting from the radiofrequency-induced hyperthermia.OBJECTIVE： To prepare nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex and to observe its growth inhibition effects on esophageal cancer cells Eca109. METHODS： The As2O3Mn0.5Zn0.5Fe2O4 complex was made using impregnation process. Its properties were described through transmission electron microscope, energy spectrometer, and atomic fluorescence spectrometer. In in vitro test, two plates of Eca109 cell lines were co-cultured with nanosized As2O3Mn0.5Zn0.5Fe2O4, Mn0.5Zn0.5Fe2O4 or aqueous of As2O3, and then one of the plates was placed in an alternating magnetic field to rise to a therapeutic temperature, which is called magnetic fluid hyperthermia. The other plate of cells was cultured as usual. RESULTS AND CONCLUSION： The nanosized As2O3/Mn0.5Zn0.5Fe2O4 were approximately global examined by transmission electron microscope. The As content measured by atomic fluorescence spectrophotometer was 0.012% to 0.066%. The proliferation ratio of the cells exposed to As2O3/Mn0.5Zn0.5Fe2O4 complex at the concentration of 5 pmol/L was significantly lower than that exposed to Mn0.5Zn0.5Fe2O4 alone or the negative control group （P 〈 0.05）. The apoptosis rate of cells exposed to As2O3Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia was much higher than that exposed alone to Mn0.5Zn0.5Fe2O4 with magnetic fluid hyperthermia or As2O3 （P 〈 0.05）. These findings indicate that AszO3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia can significantly inhibit the proliferation of esophageal cancer cells.

关 键 词：三氧化二砷 体外治疗 锰锌铁氧体 抑制肿瘤细胞 食管癌 纳米粒 复合 正常组织 

分 类 号：R733.71[医药卫生—肿瘤]