鲜壁虎活性组分对人肝癌细胞增殖和凋亡的影响  

Impact of fresh Gecko active component on proliferation and apoptosis of human hepato-cellular carcinoma cell line BEL-7404

作　　者：陆海娟[1] 罗小玲[2] 岳惠芬[2] 沈筱芸[2] 张学荣[3] 谢裕安[2] 吴继宁[2] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学附属肿瘤医院实验研究部,南宁530021 [3]广西医科大学蛇毒研究所

出　　处：《中国癌症防治杂志》2013年第1期28-31,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西教育厅科研基金资助项目(201012MS058);广西中医药管理局科研基金资助项目(GZKZ10-013)

摘　　要：目的探讨鲜壁虎活性组分对人肝癌BEL-7404细胞增殖及凋亡的影响。方法将鲜壁虎活性组分冻干粉配制为不同浓度的溶液,并作用于肝癌BEL-7404细胞。以MTT法检测不同浓度的鲜壁虎活性组分对肝癌BEL-7404细胞增殖的抑制作用,流式细胞术分析各组细胞的凋亡情况。结果不同浓度的鲜壁虎活性组分均对肝癌BEL-7404细胞的增殖有抑制作用,且呈剂量-时间依赖效应,实验组的抑制率均较对照组显著提高(P<0.05)。不同浓度的鲜壁虎活性组分均能诱导肝癌BEL-7404细胞凋亡,高浓度组(2560μg/ml)的作用更为明显(P<0.05)。结论鲜壁虎活性组分可显著抑制BEL-7404细胞的增殖,并诱导其凋亡。Objective To investigate the effects of fresh Gecko active component on proliferation and apoptosis in human hepatocel- lular carcinoma cell line BEL-7404. Methods Solutions were prepared containing different concentrations of freeze-dried fresh Gecko active component powder, and these were added to BEL-7404 cultures.MTl＂ assay was used to measure the antiproliferative effects of the different concentrations, and flow cytometry was used to assess the extent of apoptosis at the different concentrations. Results Fresh Gecko active component inhibited BEL-7404 proliferation relative to the control group（P〈0.05 ） in a time- and dose-dependent manner.The component also induced apoptosis in BEL-7404 cells,with the highest concentration（2 560 p.g/ml） showing statistically significant effects （P〈0.05）. Conclusion Fresh Gecko active component can efficiently inhibit cell proliferation and induce apoptosis in the human hepatocellular carcinoma cell line BEL-7404.

关 键 词：肝肿瘤 鲜壁虎活性组分 肝癌BEL-7404细胞 细胞增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]