金花茶抑制人食管鳞癌细胞的生长及其诱导凋亡  

Growth inhibition and apoptosis induction by Camellia chrysantha (Hu) Tuyama in human esophageal squamous carcinoma cells

作　　者：戴璐[1] 利基林 沈筱芸[2] 唐艳萍[2] 冯雁[2] 李琳[1] 张力图[2] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学附属肿瘤医院实验研究部,南宁530021

出　　处：《中国癌症防治杂志》2013年第1期32-35,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：广西卫生厅重点科研课题(重2011083);广西2011年研究生教育创新计划资助项目(2011105981002M179)

摘　　要：目的初步探讨金花茶花朵中的天然成分对人食管鳞癌细胞的生长抑制作用及其诱导细胞凋亡的情况。方法用不同浓度的金花茶花朵的水提取物作用于人食管鳞癌Eca109细胞。采用台盼蓝染色法检测金花茶花朵的水提取物对Eca109细胞活力的影响,应用透射电镜观察金花茶花朵的水提取物对癌细胞亚结构变化的作用。结果金花茶花朵的水提取物可抑制人食管鳞癌Eca109细胞的生长,其作用呈浓度和时间依赖性关系,并且能诱导细胞凋亡。结论金花茶花朵的水提取物能抑制人食管鳞癌细胞的增殖,并可诱导其凋亡,从而为金花茶作为食管癌的化学预防制剂提供实验依据。Objective To explore the ability of an aqueous extract of Camellia chrysantha（Hu） Tuyama to inhibit proliferation and induce apoptosis in human esophageal squamous cell carcinoma cells. Methods Ecal09 cells were treated with aqueous extract of Camellia chrysantha （Hu） Tuyama at various concentrations, and then cell growth was assessed by trypan blue exclusion and cell morphology by transmission electron microscopy（TEM ）. Results Aqueous extract of Camellia chrysantha （Hu） Tuyama inhibited Ecal09 growth in a dose-and time-dependent manner.The extract also induced apoptosis-like morphological changes in the cells. Conclusion These results suggest that aqueous extract of Camellia chrysantha（Hu） Tuyama inhibits cell growth and induces apoptosis in human esophageal squamous carcinoma cells.

关 键 词：食管肿瘤 金花茶花朵 生长抑制 诱导凋亡 

分 类 号：R735.1[医药卫生—肿瘤]