布洛芬对黄颡鱼Ⅰ相代谢酶及其抗氧化系统的影响  

Effects of Ibuprofen on the phase Ⅰ metabolic enzymes and antioxidant defence system of the Yellow Catfish(Pelteobagrus fulvidraco)

作　　者：武小燕[1] 彭颖[1] 廖伟[1] 李义刚[1] 苏甜[1] 欧瑞康[1] 陈昆慈[2] 李凯彬[2] 聂湘平[1] 

机构地区：[1]暨南大学生命科学技术学院生态系/水生生物研究所,广州510632 [2]中国水产科学研究院珠江水产研究所,广州510380

出　　处：《环境科学学报》2013年第4期1208-1214,共7页Acta Scientiae Circumstantiae

基　　金：国家科技支撑计划课题(No.2012BAC07B05)~~

摘　　要：使用环境相关浓度水平(0.05、0.5、5、50μg·L-1)的布洛芬(Ibuprofen,IBU)对黄颡鱼进行水体暴露,研究IBU对黄颡鱼Ⅰ相代谢酶及其抗氧化系统的影响.结果表明,随着暴露时间的延长,IBU对黄颡鱼肝脏Ⅰ相代谢P450酶系的氨基比林-N-脱甲基酶(APND)、红霉素-N-脱甲基酶(ERND)、7-乙氧基-异吩唑酮-脱乙基酶(EROD)和Ⅱ相代谢谷胱甘肽硫转移酶(GST)均表现出先诱导后抑制的作用.暴露24h时,IBU浓度为5μg·L-1实验组对I相代谢酶APND和ERND的诱导程度最大.当暴露时间达到168h时,0.5μg·L-1浓度组中APND和ERND活性均受到极显著抑制.I相代谢酶EROD活性在暴露24h后,除0.05μg·L-1浓度组无明显变化外,其它浓度组皆受到显著诱导,随着暴露时间延长到168h时,逐渐恢复到初始水平.GST活性在暴露24h后,除0.05μg·L-1浓度组外,其它浓度组均受到最大程度的诱导,过氧化氢酶(CAT)在暴露168h时被显著诱导.各浓度组丙二醛(MDA)含量在暴露24h时较对照组显著下降,72h时其含量达到最高值.其中,ERND响应较为敏感,适合作为布洛芬暴露的生物标记物.The toxic effects of ibuprofen in environmental levels of 0.05, 0.5, 5 and 50 μg·L^-1 via waterborne exposure on the phase Ⅰmetabolic enzymes and antioxidant defence system of the yellow catfish were investigated. The activities of aminopyrine N-demethylase （APND）, erythromycin N-demethylase （ERND）, 7-ethoxyresorufin -O-deethylase （EROD） and glutathione-S-transferase （GST） enzymes were induced at the initial stages and gradually inhibited with the extension of exposure time. After 24 h exposure, the highest levels of APND and ERND were detected at the concentration of 5 μg·L^-1. Obvious EROD induction was observed at 24 h in all IBU exposure groups compared to the control except 0.05 μg·L^-1 group and then returned to the normal level after 168 h. The highest value occurred at 24 h for GST and at 168 h for catalase （CAT）. The content of malondialdehyde （MDA） decreased significantly after 24 h exposure and reached the highest value at 72 h. ERND was a sensitive parameters and may be used as a potential biomarker for IBU exposure.

关 键 词：布洛芬 黄颡鱼 肝脏酶活 生物标记物 

分 类 号：X171.5[环境科学与工程—环境科学]