环氧树脂降解嗜盐菌的筛选及其处理高盐环氧树脂废水的特性研究  

Screening of Epoxy-degrading Halophiles and Their Application in High-salt Wastewater Treatment

作　　者：王进[1] 徐真[1] 彭书传[1] 夏明山[1] 岳正波[1] 陈天虎[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,合肥230009

出　　处：《环境科学》2013年第4期1510-1516,共7页Environmental Science

基　　金：安徽省科技攻关计划项目(12010402100)

摘　　要：从环氧树脂废水生化处理系统的活性污泥中分离筛选得到2株嗜盐菌,并对其进行分子生物学鉴定,考察了生长和降解特性.结果表明,通过菌株的形态观察及16S rDNA序列比对分析,菌株J1归属于芽孢杆菌属(Bacillus sp.),菌株J2属于枝芽孢杆菌属(Virgibacillus sp.).菌株J1和J2在高盐CM培养基中的适宜生长条件为:温度30℃,pH为7.0,NaCl的浓度范围为5～50 g.L-1.对环氧树脂废水中有机物的最佳降解条件为:温度30℃,pH为7.0,NaCl浓度30 g.L-1.将菌悬液J1和J2按2∶1的体积比例混合后,在复合菌种的接入量为10%时,对环氧树脂废水中COD的去除率最高.In this study,two halophilic bacteria were isolated from activated sludge in the epoxy wastewater treatment system.The strains were identified,and the growth and degradation characteristics were investigated.Strain J1 and J2 was identified respectively by morphological observation and 16S rDNA sequence alignment analysis.It was found that both strains belong to the Bacillus genus（Bacillus sp.） and branch Bacillus（Virgibacillus sp.）.The optimized growth condition of strain J1 and J2 in the high salt CM culture medium was as follows： solution temperature 30℃,pH 7.0 and 5-50 g·L-1 of NaCl.Furthermore,the best degradation condition of the organic epoxy wastewater was： temperature 30℃,pH 7.0 and NaCl concentration 30 g·L-1.When the volume ratio of bacterial suspension mixture of J1 and J2 was 2∶1 and the inoculum size of the composite strains was 10%,the highest COD removal efficiency was achieved in the epoxy wastewater treatment.

关 键 词：嗜盐菌 筛选 环氧树脂 高盐废水 鉴定 

分 类 号：X173[环境科学与工程—环境科学]