铜绿假单胞菌胞内酶粗提液对十溴联苯醚的降解  

Biodegradation of Decabromodiphenyl Ether by Intracellular Enzyme Obtained from Pseudomonas aeruginosa

作　　者：史广宇[1] 尹华[2,3] 叶锦韶[4] 彭辉[4] 张娜[4] 何宝燕[4] 

机构地区：[1]中国科学院广州地球化学研究所,广州510640 [2]华南理工大学环境科学与工程学院,广州510006 [3]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [4]暨南大学环境工程系,广州510632

出　　处：《环境科学》2013年第4期1517-1523,共7页Environmental Science

基　　金：国家自然科学基金委-广东省联合基金重点项目(U0933002);国家自然科学基金项目(50978122);广州市科技计划项目(2011J4300075)

摘　　要：探讨了铜绿假单胞菌粗酶液对十溴联苯醚(BDE-209)的降解特性,结果表明,该菌株胞内酶在12 h内对1 mg.L-1的BDE-209降解率达到了69.22%,温度、pH值、酶蛋白浓度及底物BDE-209浓度均会影响胞内酶粗提液对BDE-209的降解效率.当BDE-209浓度为1 mg.L-1时,BDE-209适宜的酶促降解条件为:温度30℃,反应pH值7.5,且降解率随着酶浓度的增加而增大.胞内酶对BDE-209的降解过程符合一级动力学反应模型,底物浓度为1 mg.L-1时降解速率最快,半衰期为6.9 h.胞内酶降解BDE-209符合高浓度底物抑制的酶促反应类型,其基本降解动力学参数为rmax=0.133 mg.(L.h)-1,Km=0.642mg.L-1,Ki=1.558 mg.L-1.The degradation characteristics of decabromodiphenyl ether（BDE-209） by crude enzyme from Pseudomonas aeruginosa were investigated.The results revealed that the degradation efficiency of the intracellular enzyme excreted from this bacterial strain reached 69.22% after incubation with 1 mg·L-1 BDE-209 for 12 h.Temperature,pH,enzyme concentration and BDE-209 concentration all influenced the ability of crude enzyme to degrade BDE-209.When the BDE-209 concentration was 1 mg·L-1,the optimal condition for enzymatic degradation was temperature 30℃ and pH 7.5,and the degradation rate increased with increasing enzyme concentration.The degradation process of BDE-209 by intracellular enzyme of the strain conformed to the first-order kinetic model.The highest reaction rate was achieved when the initial concentration of BDE-209 was 1 mg·L-1 and the half-life of this substrate was 6.9 h.In addition,the biodegradation of BDE-209 can be well described by enzymatic reaction of high concentration substrate inhibition,with a maximum substrate utilization rate of 0.133 mg·（L·h）-1,a Michaelis-Menten constant of 0.642 mg·L-1,and an inhibitory constant of 1.558 mg·L-1,respectively.

关 键 词：铜绿假单胞菌 胞内酶 十溴联苯醚 酶降解 动力学 

分 类 号：X172[环境科学与工程—环境科学]