改良型脑性瘫痪大鼠模型的建立与评估  

Establishment and assessment of modified cerebral palsy model in rats

作　　者：贺媛媛[1] 栾佐[1] 唐久来[3] 杨印祥[1] 郝庆飞[1] 杜庆安[1] 卢雅彬[1] 宁浩勇[2] 

机构地区：[1]中国人民解放军海军总医院儿科,100048 [2]中国人民解放军海军总医院病理科,100048 [3]安徽医科大学第一附属医院儿科

出　　处：《中华实用儿科临床杂志》2013年第4期304-307,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的探讨孕后期脂多糖混合溶液腹腔注射及序贯缺氧缺血对幼鼠出生28d时体质量、行为学的影响及脑组织病理学变化，评定新型脑性瘫痪动物模型构建的优势。方法孕鼠（首次怀孕10d）15只，随机取12只为实验组，孕16d时予脂多糖腹腔注射及缺氧处理，隔日1次，直至分娩，新生大鼠与母鼠为同一组，新生大鼠出生7d结扎双侧颈总动脉；对照组孕鼠于相应13期予9g／L盐水腹腔注射至分娩，分娩后新生大鼠与母鼠为同一组。2组新生大鼠监测出生28d生长发育情况，包括体质量、睁眼时间、抓力实验、旋杆实验，并行脑组织病理学检测。结果实验组与对照组新生大鼠出生体质量分别为（5．6±0．9）g和（6．2±0．8）g，睁眼时间分别为左眼（16．9±2．0）d和（13．0±2．0）d，右眼（23．1±2．6）d和（13．7±1．7）d；2组比较差异有统计学意义（P均〈0．05）抓力测试过程中得分分别为（2．7±0．8）分和（1．5±0．4）分。神经功能缺损评分分别为（4．9±2．5）分和（1．2±1．0）分，2组相比较差异均有统计学意义（P均〈0．01）。实验组和对照组旋杆实验得分分别为（1．9±0．6）分和（1．9±0．4）分，2组比较差异无统计学意义（P=0．871）。实验组脑组织病理切片HE染色显示神经元排列紊乱，丢失，白质变薄，脑实质有炎性细胞浸润，并可见到明显细胞海绵状变性；对照组灰质层清晰，神经元规则排列，灰白质界限清楚。结论孕后期予脂多糖腹腔注射及序贯缺氧缺血能构建稳定的脑性瘫痪模型。Objective To construct a novel type of cerebral palsy model in rats with intraperitoneal injection of fipopolysaecharide（LPS） and sequential oxygen deficit from pregnancy 16＇h day to the end of pregnancy and chronic bilateral common carotid artery occlusion on the 28th day. Assessing by weight, behaviour, modified neurological severity score, and pathological changes and to explain the superiority of our model. Methods Twelve SD pregnant rats were randomly selected as the experimental group ,3 SD pregnant rats were selected as the control group. From 16 days of pregnant, the pregnant rats were injected with LPS by intraperitoneal injection, and received oxygen deficit in the experimental group, and the pregnant rats were received saline solution in the control group until delivery. On the postnatal 7th day, bilateral common carotid arteries were ligated in the experimental group. The weight, eye-opening time, grip test, rotarod test and pathological section of the brain of newborn rats in the both groups were detected. Results In the experimental group and the control group, the weight were （ 5.6 ± 0.9 ） g and （ 6.2 ± 0.8 ） g, the left eyes open day were （ 16.9 ± 2.0） d and （ 13.0 ± 2.0） d, the right eyes were （23.1 ± 2.6） d and （ 13.7 ± 1.7 ） d, respectively, there were significant differences between the both groups （ all P 〈 0.05 ）. In the experimental group and the control group, the score in grip test were （2.7 ±0.8） scores and （1.5 ±0.4） scores and MNSS were （4.9 ±2.5） scores and （ 1.2 ± 1.0） scores, there were significant differences （ all P 〈 0.01 ）. Neurons arrange in disorder, the white matter is thinner and inflammatory cells infiltrated in the experimental group pups. However in control group, brain tissue pathology slices showed neurons in neat rows, the gray and write matters boundaries were clear, and the corpus callosum was mature. Conclusions In late pregnancy intraperitoneal injection LPS and hypoxia with postnatal bilateral com

关 键 词：脑损伤 感染 脑性瘫痪 缺氧缺血 行为学 

分 类 号：R-332[医药卫生] R742