p75^(NTR)、D2-40和P63在乳腺癌中的表达及HER-2基因检测中的应用  

The application of p75^(NTR), D2-40 and P63 in the expression of breast cancer and detection of HER-2 gene

作　　者：钟惠玲 吴叶菊[2] 

机构地区：[1]新疆克拉玛依市人民医院病理科,克拉玛依834000 [2]新疆克拉玛依市中心医院病理科

出　　处：《现代预防医学》2013年第7期1316-1319,1322,共5页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30760072)

摘　　要：目的探讨p75NTR、D2-40和P63肌上皮标记物在乳腺纤维腺瘤、导管内癌和浸润性导管癌中的表达,鉴别乳腺癌组织中浸润性导管癌与导管内癌并检测其HER-2基因状态。方法用EnVision法对68例乳腺纤维腺瘤、导管内癌和浸润性导管癌分别进行p75NTR、D2-40和P63免疫染色,用FISH技术检测28例乳腺癌组织中浸润性导管癌和导管内癌的HER-2基因状态。结果 p75NTR、D2-40、P63在乳腺纤维腺瘤与导管原位癌中的表达差异没有统计学意义,在导管原位癌与浸润性导管癌中的表达差异有统计学意义。同时对28例乳腺癌组织切片上的浸润癌和导管内癌部分进行HER-2基因检测,二者HER-2基因状态的一致率为7.14%(2/28)。结论联合使用p75NTR、P63和D2-40是比较理想的乳腺肌上皮细胞的标记物,应用这些抗体保证检测乳腺浸润性导管癌HER-2基因的准确性。OBJECTIVE To investigate the expressions of markers of myoepithelial cells （p75NTR, D2-40 and P63） in fibroadenoma of breast, ductal carcinoma in situ （DCIS） and invasive ductal carcinoma （IDCA）. And to make a distinction between the DCIS and IDCA in the same breast organizations and test the expressions of HER-2. METHODS Detected the expression lever of p75NTR, D2-40 and P63 in 68 cases of fibroadenoma of breast, DCIS and IDCA by immunohistochemistry; and detected the expression of HER-2 gene in 28 cases of DCIS and IDCA by FISH. RESULTS The differences in the expression of p75NTR, D2-40 and P63 between the fibroadenoma of breast and DCIS had no statistical significance. But it was meaningful between DCIS and IDCA. Detected the expression of HER-2 gene in 28 cases of DCIS and IDCA at the same time, the concordance rate of HER-2 gene expression in the DCIS and IDCA was 7.14% （2 / 28）. CONCLUSION The combination of p75NTR, D2-40 and P63 is ideal marker of myoepithelial cells, which can help us to ensure the accuracy of the HER-2 gene detection.

关 键 词：乳腺癌 肌上皮细胞 p75NTR D2-40 P63 HER-2基因 

分 类 号：R737.9[医药卫生—肿瘤]