玉米穗位高全基因组关联分析及其候选基因预测  

Genome-Wide Association Study and Candidate Gene Prediction of Ear Height in Maize (Zea mays L.)

作　　者：李博[1] 张焕欣[2] 杨小艳[3] 吕颖颖[1] 江培顺[1] 郝转芳[2] 吕香玲[1] 王宏伟[1] 翁建峰[2] 

机构地区：[1]沈阳农业大学农学院,辽宁沈阳110000 [2]中国农业科学院作物科学研究所/作物分子育种国家工程实验室,北京100081 [3]重庆市农业科学院生物技术研究中心,重庆404100

出　　处：《作物杂志》2013年第2期27-32,共6页Crops

基　　金：国家自然科学基金项目(31201219);973项目(2011CB100106)

摘　　要：利用41101个SNP标记对159份我国玉米自交系进行全基因组分析。通过控制群体结构和亲缘关系,采取混合线性模型,检测到7个与穗位高显著相关SNP(P<0.0001),并确定7个候选基因,其注释功能有GABA-B受体活性、丝氨酸/苏氨酸蛋白激酶活性、组蛋白-赖氨酸N-甲基转移酶活性、转运活性、翻译起始因子活性和酰胺基tRNA水解酶活性。利用BioMercator2-1软件将收集的137个穗位高QTL整合至IBM2 2008Neighbors玉米遗传图谱上,确定19个"一致性"QTL,结合分析7个与穗位高显著相关的SNP位点信息,获得2个候选基因。With the population structure and kinship controlled in a mixed linear model （ MLM）, a total of 159 Chinese maize inbred lines and 41101 single-nucleotide polymorphism （SNP） markers were used to perform the ge- nome-wide association study. A total of seven SNPs were found to be significantly associated with ear height at P 〈 0.0001 ,and seven candidate genes were identified with annotated functions of GABA-B receptor activity, protein serine/threonine kinase activity, histone-lysine N-methyltransferase activity, transporter activity, translation initiation factor activity and aminoacyl-tRNA hydrolase activity, respectively. A total of 137 QTL for ear height were integrated to IBM2 2008 neighbors maize genetic map by BioMercator2-1 software, and 19 ＂Consensus＂ QTL were identified. Combining analysis of the seven significant SNPs and 19 ＂Consensus＂ OTL.two candidate genes were identified.

关 键 词：玉米 穗位高 全基因组关联分析 元分析 

分 类 号：S513[农业科学—作物学]