亚低温在全脑缺血再灌注损伤中对大鼠海马NO合成的影响  

Effect of Mild Hypothermia on Nitric Oxide Synthesis in Hippocampus during Reperftision after Global Cerebral Ischemia in Rats

作　　者：徐海涛[1] 叶应湖[1] 黄书岚[1] 王国安[1] 陈坚[1] 刘胜[1] 戴宝生 

机构地区：[1]湖北医科大学附属第一医院神经外科,武汉430060

出　　处：《中国临床神经外科杂志》2000年第2期100-102,共3页Chinese Journal of Clinical Neurosurgery

摘　　要：目的 通过研究亚低温对NO合成的影响,探讨亚低温脑保护效应的机制。方法 采用4-VO法制作全脑缺血模型。动物分为8组,均缺血12min,于再灌注30min、60min、2h、4h后处死取材,测定海马区NO含量及NOS活性。结果 ①低温30及60min组NO含量较相应常温组显著降低(P<0.01),NOS活性亦显著降低(P<0.05);②常温30min组NO含量、NOS活性较高,60min组达到高峰,以后逐渐下降;③NO含量与NOS活性呈显著正相关(r=0.76,P<0.01)。结论 亚低温抑制海马NO的合成,可能是亚低温脑保护的机制之一。Objective To investigate possible neuroprotecrive machanism of hypothermia, the effect of mild hypothermia on the synthesis of nitric oxide (NO) was observed in the hippocampus of rats during reperfusion after global cerebral ischemia. Methods Global cerebral ischemia was produced according to the model of Pulsineli. Temporal muscle temperatures were monitored continuously. Forty adult SD rats were divided randomly into eight groups. All rats were subjected to 12 min of ischemia. These rats both in the normothermic groups (37-381) and hypothermic groups (32-33℃) were sacrisfied 30 min, 60 min, 2 hours and 4 hours after ischemia and the NO level and NOS (nitric oxide synthase) activity in the hippocampus were measured respectively. Results (1)The NO level and the NOS activity in the hypothermic groups which underwent 30 min, 60 min of the reperfusion were significantly lower than those in the corresponding normothermic groups ( P<0.01, 0.05 respectively). (2)In the normothermic groups, the NO level and the NOS activity were relatively high 30 min after the ischemia and reached to the peak at 60 min, then dropped gradually. (3)It was found that the NO level correlated positively with the NOS activity (r = 0.76, P <0.01). Conclusions The authors concluded that, the mild hypothermia could inhibit nitric oxide synthesis after the global cerebral ischemia and this might be one of its protective machanisms during reperfusion.

关 键 词：大鼠 脑损伤 低温 人工 一氧化氮 

分 类 号：R363.123[医药卫生—病理学]