黄芪多糖对大鼠口腔溃疡治疗作用研究  

Therapeutic Effects of Astragalus Polysaccharides on Oral Ulcers in Rats

作　　者：王雪梅[1] 薄磊[1] 祁晶[1] 余占海[1] 马兴铭[2] 

机构地区：[1]兰州大学口腔医学院 [2]兰州大学基础医学院,兰州730000

出　　处：《天然产物研究与开发》2013年第3期321-324,共4页Natural Product Research and Development

摘　　要：本研究旨在探讨黄芪多糖对患有口腔溃疡大鼠(recurrent aphthous ulcer,RAU)的表皮细胞生长因子(epidermal growth factor,EGF)水平及T淋巴细胞亚群的影响。根据溃疡面的直径计算愈合率及HE染色观察溃疡组织病理学改变,酶联免疫吸附法(ELISA)测定EGF的水平,流式细胞仪检测大鼠外周血CD4+T细胞、CD8+T细胞亚群的百分率。实验结果显示治疗20 d后,黄芪多糖组大鼠溃疡面积减小,愈合率为87.5%～100%。与正常对照组相比,模型对照组大鼠CD4+T细胞数减少、CD8+T细胞数升高,CD4+/CD8+比值降低(P<0.05)。与模型对照组相比,黄芪多糖组大鼠外周血CD4+T细胞数升高、CD8+T细胞数降低,CD4+/CD8+比值回升(P<0.05)。EGF水平在造模后出现明显下降,黄芪多糖灌胃治疗后有升高。提示黄芪多糖能够提高RAU实验性大鼠EGF的水平和改善T细胞亚群的失衡状态,具有明显地促进口腔溃疡愈合的作用。The objective of this study was to investigate the influence of astragalus polysaccharide （APS） on rat aphthous ulcers （recurrent aphthous ulcer, RAU） epidermal growth factor （EGF） and on T-lymphocyte subsets. Calculating healing rate according to the diameter of ulcer, observing pathological changes of ulcer tissue using HE chromosome, measuring EGF level by ELISA method, measuring peripheral blood CD4^＋ T cells of rats and percentage of CD8 ^＋T subsets cells. In 20 days treatment,APS reduced the ulcer area in rats ,the healing rate reached 87.5% to 100%. Compared with the control group ,model control group rats reduced the number of CD4^＋ T ceils, CD8 ＋ T cells increased, CD4^＋/CD8 ^＋ratio decreased （P 〈 0.05）. Compared with the control group, APS peripheral blood of rats increased the number of CD4^＋T cells, CD8 ^＋T ceils decreased, CD4^＋/CD8^＋ ratio rose （ P 〈 0.05 ）. The EGF level decreased significantly after modeling, but re-increased after filling stomach treatment, indicating that APS can increase the EGF level of RAU rat and can improve the imbalance of T cell subsets,with an obvious promotion of oral, ulcer healing.

关 键 词：口腔溃疡 黄芪多糖 EGF T淋巴细胞 大鼠 

分 类 号：R781[医药卫生—口腔医学]