腹膜乳斑与胃癌细胞在腹膜播散中的关系  

The relationship between peritoneal milky spots and peritoneal dissemination of gastric carcinoma

作　　者：安伟德[1] 胡祥[1] 梁品[1] 吴昊[1] 沈忠义[1] 

机构地区：[1]大连医科大学第一临床学院普通外科,116011

出　　处：《中国胃肠外科杂志》2000年第3期172-174,共3页

基　　金：辽宁省自然科学基金资助项目!(9810500106)

摘　　要：目的研究腹膜乳斑与胃癌细胞在腹膜播散中的关系 .方法 30只经环孢素 A处理的 SD大鼠分成 3组 ,分别向大鼠腹腔内接种经溴脱氧尿甙 (BrdU)标记的 Sy86B裸鼠可移植性人胃癌细胞 ,数量分别为 105、 106和 107.应用免疫组织化学染色 ,观察癌细胞的分布 .计数网膜乳斑或非乳斑区标记细胞的数量 ,确定癌细胞的分布 .结果在乳斑或非乳斑区平均标记细胞数的比率分别是 163∶ 1(105)、 280∶ 1(106)和 697∶ 1(107).标记细胞数在乳斑或非乳斑区之间的差异有显著性意义 (P< 0 01).结论标记细胞选择性地侵入网膜乳斑 ,表明在胃癌腹膜播散的早期 ,癌细胞具有特异地侵入腹膜乳斑的特性 .Objective To investigate the relationship between peritoneal milky spots and peritoneal dissemination of gastric carcinoma. Methods Thirty SD rats treated with cyclosporin A were divided into 3 groups and inoculated introperitoneally with human gastric cancer sy86B cells labeled with bromodeoxyuridine(BrdU)at 105、 106 and 107 cells per rat.The distribution of the labeled cells in the greater omentum,taken from the rats using an anti- BrdU antibody,was observed with immunohistochemical assay.The number of the BrdU- labeled cell per unit area at each milky spot and non- milky spot in the omentum was counted.Results The ratios of the mean labeled cell numbers in the milky spot sites to that in the non- milky spot sites were 163∶ 1(105), 280∶ 1(106) and 697∶ 1(107)respectively.There were statistically significant differences of the numbers of BrdU- labeled cell@ mm－ 2 between milky spot sites and non- milky spot sites.Conclusion Labeled cancer cells spreading in the peritoneal cavity infiltrate selectively milky spots in the early stage of peritoneal dissemination of gastric carcinoma.

关 键 词：胃肿瘤 肿瘤转移 腹膜 乳斑 

分 类 号：R735.2[医药卫生—肿瘤]