兔脂肪肝及正常肝内炎性假瘤模型制备及其超声表现  

Establishment of Inflammatory Pseudotumor of Liver modified model in rabbits with fatty liver and normal liver showed on ultrasound imaging

作　　者：张炎晶[1] 刘利平[1] 鲁琴[1] 赵育芳[1] 路喜安[2] 郝艳红[1] 徐琨[1] 曹成[1] 杨婧[1] 

机构地区：[1]山西医科大学第一医院超声科,太原030001 [2]山西医科大学第一医院病理科,太原030001

出　　处：《中国医疗前沿》2013年第5期27-28,共2页China Healthcare Innovation

基　　金：国家自然科学基金面上项目(编号:81171361);山西省人事厅留学人员择优资助项目(编号:2011-762)

摘　　要：目的建立一种可供研究的兔脂肪肝及正常肝内肝炎性假瘤(IPL)模型,研究其超声表现,以便更好的服务临床。方法兔脂肪肝模型的建立:高脂饲料+5%的酒精喂养,4周;兔肝炎性假瘤的建立:用改良的注射方法将弗氏佐剂分别注入兔肝左右叶,分别于1周、2周、3周动态观察兔肝内病灶的超声表现,最后取标本做病理检查。结果⑴脂肪肝及正常肝内IPL的成功率均为100%。⑵常规超声及病理对照:①脂肪肝饮食4周后,肝实质回声细腻增强,病理表现为肝细胞不同程度的脂肪变性;②造模1周及2周的IPL病灶周围肝组织回声增强,增粗,病灶内部坏死区内残留不同程度未吸收的明胶海绵(造模时植入);③造模3周的IPL病灶周围肝实质回声均匀,病灶内部坏死区内不含有未吸收的明胶海绵,病理学上更接近临床IPL。结论通过观察兔脂肪肝及正常肝内IPL模型的动态形成过程,发现兔IPL建模3周为最佳,为进一步探讨IPL的形成机制及不同肝背景下IPL的影像学研究发挥了积极作用。Objective To establish a model carrying IPL in rabbits serving for clinical study and to analyse its ultrasound features.Methods Establishment of rabbit models of fatty liver： High fat diet＋5% alcohol for 4 weeks;Establishment of rabbit models of IPL： Freund＇s complete adjuvant was injected with modified methods into the desirable portion of the liver in rabbits.In 1 week,2 weeks,3 weeks after the injection,all animals were examined on ultrasound and the images were analyzed.Then,the lesions were harvested for future pathological examination.Results ⑴The incidence rate of IPL was 100%.⑵Ultrasound and Pathology： ①Fatty liver diet for 4 weeks,liver echo is delicate enhancement,hepatic cells are fatty change in different degrees in pathology;②Molding after 1and 2 weeks,liver echo is enhancement and thickening around the lesions.Necrotic area have gelatin sponge unabsorbed.③ Molding after 3weeks,liver echo is well-distributed around the lesions.Necrotic area don＇t have gelatin sponge,and pathology is close to clinical.Conclusions By dynamically observing the formation process of IPL rabbit model of fatty liver and normal liver.Fand the best time for IPLmodel is 3 weeks,It＇s better for inquire into formation mechanism of IPL.And imaging studies of the IPL in different hepatic background.

关 键 词：兔肝炎性假瘤 动物模型 超声 

分 类 号：R364.2[医药卫生—病理学]