维生素C对重度失血性休克大鼠早期口服补液时胃缺血再灌注损伤的影响  被引量:1

Vitamin C alleviates gastric ischemic-reperfusion injury during oral fluid resuscitation of severe hemorrhagic shock rats

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作  者:周国勇 刘真 贾赤宇 

机构地区:[1]解放军第三○九医院烧伤整形科,北京100091

出  处:《中华损伤与修复杂志(电子版)》2012年第5期13-16,共4页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

摘  要:目的研究口服维生素C对40%血容量失血休克大鼠口服补液时胃缺血再灌注损伤的影响。方法雄性SD大鼠56只,氯胺酮-速眠新Ⅱ肌注复合麻醉后,行右侧颈总动脉插管,按全身血容量的40%分两次间隔15min放血。随机分为假手术组(S组,n=8)、单纯失血组(H组,n=16)、失血+葡萄糖-电解质溶液组(H+GES,n=16)、失血+葡萄糖-电解质溶液/维生素C组(H+GES/VC组,n=16)。H+GES组和H+GES/VC组大鼠在失血后0.5h及1h分别给予2倍于失血总量的葡萄糖-电解质溶液。H+GES/VC组将维生素C(250mg/kg)溶于葡萄糖-电解质溶液,于首次灌胃时一次给入。H、H+GES、H+GES/VC3组分别于放血后2h及4h各处死大鼠8只,取胃组织测定丙二醛(MDA)含量、髓过氧化物酶(MPO)和谷胱甘肽(GSH)活性,干湿重法测定胃组织含水率(GW)。结果失血后2h和4h,H+GES组胃组织MDA含量和MPO活力均显著高于H组,GSH活性较H组分别低49.5%和53.2%,差异具有统计学意义(P<0.05);而H+GES/VC组2h和4hMDA含量和MPO活力均较低于H+GES组,GSH活性较H+GES组分别高28.7%和67.5%,差异具有统计学意义(P<0.05)。与H组胃组织GW(67.69±4.36)%相比,H+GES组胃组织GW(82.10±4.01)%增加,差异具有统计学意义(P<0.05),而H+GES/VC组胃组织GW(75.11±3.90)%低于H+GES组,差异具有统计学意义(P<0.05)。结论维生素C能减轻40%血容量失血大鼠口服补液时胃缺血再灌注损伤,减少口服补液时的胃肠道并发症。Objective To investigate the effect of vitamin C (VC) on gastric ischemic/reperfusion (I/R) injury during oral fluid resuscitation of hemorrhagic shock in rats with 40% blood volume loss. Methods Fifty six SD rats were subjected to a hemorrhagic shock induced by a 40% blood volume loss from right common carotid artery and divided randomly into four groups : operation shame ( S group, n = 8 ) , hemorrhage with no resuscitation (H group, n = 16), hemorrhage with oral infusion of either a glucose- electrolyte solution (H + GES, n = 16) or GES containing vitamin C ( H + GES/VC, n = 16). GES was orally administrated in an total amount double of blood loss in 0.5 h and 1 h after bleeding. Vitamin C ( 250 mg/kg) was administered together with GES in the first lavage. Rats were sacrificed 2 h or 4 h afterhemorrhage and specimens of stomach were harvested for evaluation of malondialdehyde ( MDA), glutathione (GSH) and myeloperoxidase (MPO). Gastric water contents were determined by ratios of dry to wet weight. Results Compared to those of H group, oral resuscitation with GES led to significant increase of MDA content and MPO activity in gastric tissue of both 2 h and 4 h after bleeding ( P 〈 0.05 ) , while GSH activity of 2 h and 4 h after bleeding in H + GES group was lowered by 49.5% ,53.2% ( P 〈 0.05 ). And GES/VC administration resulted in markedly lower levels of MPO and MDA (P 〈 0.05 ) of H + GES/VC group in both 2 h and 4 h after bleeding compared to those of H + GES group, while GSH activity hightened respectively 28.7% and 67.5%. Gastric water contents of H + GES group[ (82.1 ± 4.01 ) % ] was higher than that of H group[(67.69±4.36)%] (P〈0.05) and H+GES/VC group [(75.11 ±3.90)%], lower than GES group (P 〈 0.05 ). Conclusion The results indicated that VC alleviated gastric ischemia-reperfusion injury and inhibited the development of complication of oral fluid resuscitation in hemorrhagic shock rats.

关 键 词:抗坏血酸 失血 补液疗法  再灌注损伤 

分 类 号:R605.971[医药卫生—急诊医学]

 

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