p51基因在鼻咽癌组织中表达的研究  

The expression of p51A and p51B gene in nasopharyngeal carcinoma

作　　者：李虹[1] 张玲[1] 姚开泰[1] 

机构地区：[1]湖南医科大学肿瘤研究所卫生部重点实验室,湖南长沙410078

出　　处：《中国病理生理杂志》2000年第9期769-774,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金重点资助 !(39730200)

摘　　要：目的 :研究p5 1基因的两个转录本p5 1A和p5 1B在鼻咽癌及对照组织中的表达谱 ,为进一步探讨p5 1在鼻咽癌癌变过程中的作用打下初步基础。方法 :(1)用PCR -SSCP银染技术检测 2 1例鼻咽癌组织及配对外周血标本p5 3基因的突变。 (2 )用RT -PCR检测p5 1A和p5 1B在鼻咽癌及对照组织中的表达。结果 :(1) 2 1例鼻咽癌组织及配对外周血标本p5 3基因未发现突变。 (2 )在鼻咽癌及对照组织中 ,p5 1B的mRNA表达要高于p5 1A。 (3)p5 1A和p5 1BmRNA在鼻咽癌组织中的表达高于对照组织。结论 :(1)p5 1A和p5 1BmRNA在鼻咽癌中的表达高于对照组织。 (2 )p5 1A和p5 1BmRNA表达的改变与p5AIM: To investigate the expression map of two transcripts, p51A and p51B of p51 gene in nasopharyngeal carcinoma (NPC). METHODS: p53 gene mutation of 21 NPC tissue and blood are analyzed by PCR-SSCP silver stain technique. The expression of p51A and p51B mRNA in NPC tissue and control group are tested by RT-PCR.RESULTS: No gene mutation of p53 is discovered on exon 5-8. p51B mRNA shows higher expression than p51A. The expression of p51B and p51A mRNA on NPC tissue is much higher than that in control group. CONCLUSION: There are differential expression of p51A and p51B mRNA between NPC tissue and control group. The alteration expression of p51A and p51B show no relationship with p53 gene mutation.

关 键 词：鼻咽肿瘤 基因表达 P51基因 免疫组织化学 MRNA 

分 类 号：R739.630.2[医药卫生—肿瘤]