血管生成素-2和血管内皮生长因子在酸中毒诱导早产儿视网膜病的大鼠动物模型中的表达  

Expression of VEGF and Ang-2 in a rat model of acidosis-induced retinopathy of prematuirity

作　　者：侯玮玮[1] 朱玲玲[1] 刘永春[1] 陶月红[1] 

机构地区：[1]江苏省苏北人民医院儿科,江苏扬州225001

出　　处：《实用临床医药杂志》2013年第3期5-8,共4页Journal of Clinical Medicine in Practice

摘　　要：目的建立酸中毒诱导的早产儿视网膜病(ROP)大鼠动物模型(AIR模型),研究血管内皮生长因子(VEGF)、血管生成素-2(Ang-2)的表达变化规律,探讨其与ROP血管发育及新生血管形成之间的关系。方法 1日龄SD新生大鼠随机分为酸中毒诱导模型组(ROP组)和正常对照组(C组),通过管饲NH4Cl方法建立AIR模型。应用免疫组化方法标记视网膜VEGF、Ang-2的表达水平。结果免疫组化结果显示,8日龄ROP大鼠VEGF、Ang-2的表达水平明显低于对照组(P<0.05),而在停止管饲NH4Cl后的10日龄、13日龄ROP组大鼠视网膜VEGF、Ang-2表达水平明显高于对照组(P<0.05)。在实验过程中VEGF及Ang-2的表达趋势相同。结论酸中毒使VEGF及Ang-2的蛋白表达水平下调;停止管饲NH4Cl后2者表达水平上调;2者变化过程与视网膜新生血管发展进程有关。VEGF、Ang-2可能在视网膜正常血管发育及ROP的新生血管化进程中起到重要作用。Objective To establish an animal model of acidosis-induced retinopathy （ AIR）, analyze the expression of vascular endothelial growth factor （VEGF） and angiopoietin-2 （Ang-2） in a rat model of acidosis-induced retinopathy and to evaluate the relationship between VEGF, Ang-2 and neovascularizafion（NV）. Methods Newborn Sprague-Dawley rats were randomly divided into acidosis-induced retinopathy group（ ROP group） and control group. VEGF and Ang-2 in retina were detected by immunohistochemistry. Results The result of immunohistochemistry revealed that the protein expression of VEGF and Ang-2 of the ROP group on the 8th day was lower than that of the control group （P 〈0.05） and the protein expressions of VEGF and Ang-2 on the 10th and 13th day were stronger than those of the control group （P 〈 0.05）. The changes of VEGF and Ang-2 protein expression were the same. Conclusion The periods of acidosis result in down-regulation of VEGF and Ang- 2. When acidosis stops, the expression of VEGF and Ang-2 is up-regulated. The abnormal retina VEGF and Ang-2 expression may play an important role in the development of neovascularization in ROP.

关 键 词：视网膜病 血管内皮生长因子 血管生成素-2 视网膜新生血管 大鼠 

分 类 号：R774.1[医药卫生—眼科]