贲门癌患者外周血人类端粒酶反转录酶及基质金属蛋白酶-7mRNA表达的临床意义  

Clinical significance of the mRNA expressions of human telomerase tranacriptase and MMP-7 in the peripheral blood of patients with cardia cancer

作　　者：岳强[1] 杨斌[1] 王春艳[3] 吴乾[2] 郑光华[1] 张荣生[1] 张羽捷[1] 

机构地区：[1]山西省肿瘤医院胸外科三病区,太原030013 [2]山西省肿瘤医院胸外科中医科,太原030013 [3]山西省肿瘤研究所分子生物室

出　　处：《肿瘤研究与临床》2013年第3期166-168,172,共4页Cancer Research and Clinic

摘　　要：目的分析人类端粒酶反转录酶（hTERT）及基质金属蛋白酶-7（MMP-7）mRNA在贲门癌患者外周血中的表达。方法采用实时荧光定量反转录-聚合酶链反应（RT—PCR）法检测157例贲门癌患者术前血液标本中MMP．7mRNA和hTERTmRNA表达，对其表达与临床病理参数的关系进行比较。对无转移的81例患者术后6、12个月进行随访，根据患者临床症状、体征相应检查，判断复发转移。并将MMP-7mRNA和hTERTmRNA表达阳性组与阴性组复发转移情况进行比较。结果157例贲门癌患者中，hTERT阳性表达73例（46．0％）；MMP．7阳性表达61例（38．8％）。hTERT、MMP-7表达与性别（X^2值为2．597、0．199）、年龄（X^2值为4．314、0．073）等因素无相关性（均P〉0．05），但与TNM分期（X^2值为10．624、14．530）、有无远处转移（X^2值为7．294、12．824）及组织的分化程度（X^2值为12．003、6．482）之间存在相关性（均P〈0．05）。术后随访6、12个月肿瘤转移率比较差异有统计学意义（P〈0．05）。血行微转移阳性患者术后12个月肿瘤转移概率是阴性患者的6．12倍（OR=6．120）。结论贲门癌患者外周血hTERT及MMP-7mRNA的异常表达与贲门癌的发展和转移有关，血液微转移检测对贲门癌早期发现及预后具有重要意义，值得进-步研究。Objective To detect the expressions of human telomerase tranacriptase （hTERT） and MMP-7 mRNA in the peripheral blood of patients with cardia cancer. Methods Total RNA was extracted with TRIzol and was transcribed reversely into cDNA. RT-PCR was used to detect mRNA expression of MMP-7 and hTERT in the peripheral blood of 157 patients with cardia cancer. 81 patients without metastasis were followed up for 12 months. Results Of 157 patients, 73 cases （46.0 %） and 61 cases （38.8 %） had the positive expressions of hTERT, MMP-7, respectively. There were significant correlations between the blood micro-metastases MMP-7 and hTERT expressions and TNM staging （X2 = 10.624, 14.530）, tumor metastasis （X2 = 7.294, 12.824）, and differentiation degree （9（2 = 12.003, 6.482）, but no significant correlations were seen in sex （X2 = 2.597, 0.199） and age （X2 = 4.314, 0.073）. There was statistically significant in the occurrence rate of tumor metastasis between 6-month and 12-month follow-up. The occurrence rate of metastasis in patients with micro-metastases was 6.12-fold compared with patients without micro-metastases after 12-month follow-up. Conclusion Detection of MMP-7 and hTERT expressions can be used as reliable markers to predict peripheral blood micro-metastasis in patients with cardia carcinoma.

关 键 词：胃肿瘤 人类端粒酶反转录酶 基质金属蛋白酶类 反转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]